Imperial College London
Portrait Picture

Professor José R Penadés

Faculty of Medicine, Department of Infectious Disease

Director MRC Centre for Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 8533j.penades Website

 
 
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Assistant

 

Mrs Anna Lee +44 (0)20 7594 2954

 
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Location

 

Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ibarra-Chávez:2020:2020/5783064,
author = {Ibarra-Chávez, R and Haag, AF and Dorado-Morales, P and Lasa, I and Penadés, JR},
doi = {2020/5783064},
journal = {BioDesign Research},
pages = {1--14},
title = {Rebooting synthetic phage-inducible chromosomal islands: one method to forge them all},
url = {http://dx.doi.org/10.34133/2020/5783064},
volume = {2020},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Phage-inducible chromosomal islands (PICIs) are a widespread family of mobile genetic elements, which have an important role in bacterial pathogenesis. These elements mobilize among bacterial species at extremely high frequencies, representing an attractive tool for the delivery of synthetic genes. However, tools for their genetic manipulation are limited and timing consuming. Here, we have adapted a synthetic biology approach for rapidly editing of PICIs in Saccharomyces cerevisiae based on their ability to excise and integrate into the bacterial chromosome of their cognate host species. As proof of concept, we engineered several PICIs from Staphylococcus aureus and Escherichia coli and validated this methodology for the study of the biology of these elements by generating multiple and simultaneous mutations in different PICI genes. For biotechnological purposes, we also synthetically constructed PICIs as Trojan horses to deliver different CRISPR-Cas9 systems designed to either cure plasmids or eliminate cells carrying the targeted genes. Our results demonstrate that the strategy developed here can be employed universally to study PICIs and enable new approaches for diagnosis and treatment of bacterial diseases.
AU  - Ibarra-Chávez,R
AU  - Haag,AF
AU  - Dorado-Morales,P
AU  - Lasa,I
AU  - Penadés,JR
DO  - 2020/5783064
EP  - 14
PY  - 2020///
SP  - 1
TI  - Rebooting synthetic phage-inducible chromosomal islands: one method to forge them all
T2  - BioDesign Research
UR  - http://dx.doi.org/10.34133/2020/5783064
UR  - https://spj.sciencemag.org/journals/bdr/2020/5783064/
UR  - http://hdl.handle.net/10044/1/84167
VL  - 2020
ER  -
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