Imperial College London

DrJesusRodriguez Manzano

Faculty of MedicineDepartment of Infectious Disease

Non-Clinical Lecturer in Antimicrobial Resistance and Infect
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Electrical EngineeringSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Malpartida-Cardenas:2019:10.1101/638221,
author = {Malpartida-Cardenas, K and Miscourides, N and Rodriguez-Manzano, J and Yu, LS and Baum, J and Georgiou, P},
doi = {10.1101/638221},
publisher = {Cold Spring Harbor Laboratory},
title = {Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform},
url = {http://dx.doi.org/10.1101/638221},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - UNPB
AB - <jats:title>Abstract</jats:title><jats:p>Early and accurate diagnosis of malaria and drug-resistance is essential to effective disease management. Available rapid malaria diagnostic tests present limitations in analytical sensitivity, drug-resistant testing and/or quantification. Conversely, diagnostic methods based on nucleic acid amplification stepped forwards owing to their high sensitivity, specificity and robustness. Nevertheless, these methods commonly rely on optical measurements and complex instrumentation which limit their applicability in resource-poor, point-of-care settings. This paper reports the specific, quantitative and fully-electronic detection of <jats:italic>Plas-modium falciparum</jats:italic>, the predominant malaria-causing parasite worldwide, using a Lab-on-Chip platform developed in-house. Furthermore, we demonstrate on-chip detection of C580Y, the most prevalent single-nucleotide polymorphism associated to artemisinin-resistant malaria. Real-time non-optical DNA sensing is facilitated using Ion-Sensitive Field-Effect Transistors, fabricated in unmodified complementary metal-oxide-semiconductor technology, coupled with loop-mediated isothermal amplification. This work holds significant potential for the development of a fully portable and quantitative malaria diagnostic that can be used as a rapid point-of-care test.</jats:p>
AU - Malpartida-Cardenas,K
AU - Miscourides,N
AU - Rodriguez-Manzano,J
AU - Yu,LS
AU - Baum,J
AU - Georgiou,P
DO - 10.1101/638221
PB - Cold Spring Harbor Laboratory
PY - 2019///
TI - Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
UR - http://dx.doi.org/10.1101/638221
ER -