Imperial College London

DrJesusRodriguez Manzano

Faculty of MedicineDepartment of Infectious Disease

Non-Clinical Lecturer in Antimicrobial Resistance and Infect
 
 
 
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Contact

 

j.rodriguez-manzano

 
 
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Location

 

Electrical EngineeringSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rodriguez-Manzano:2018:10.1101/409912,
author = {Rodriguez-Manzano, J and Moniri, A and Malpartida-Cardenas, K and Dronavalli, J and Davies, F and Holmes, A and Georgiou, P},
doi = {10.1101/409912},
title = {Simultaneous single-channel multiplex and quantification of carbapenem-resistant genes using multidimensional standard curves},
url = {http://dx.doi.org/10.1101/409912},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - <jats:title>ABSTRACT</jats:title><jats:p>Multiplexing and absolute quantification of nucleic acids, both have, in their own right, significant and extensive use in biomedical related fields, especially in point-of-care applications. Currently, the ability to detect several nucleic acid targets in a single-reaction scales linearly with the number of targets; an expensive and time-consuming feat. Here, we propose a new methodology based on multidimensional standard curves that extends the use of real-time PCR data obtained by common qPCR instruments. By applying this novel methodology, we achieve simultaneous single-channel multiplexing and enhanced quantification of multiple targets using only real-time amplification data. This is obtained without the need of fluorescent probes, agarose gels, melting curves or sequencing analysis. Given the importance and demand for tackling challenges in antimicrobial resistance, the proposed method is applied to the four most prominent carbapenem-resistant genes: <jats:italic>bla</jats:italic><jats:sub>OXA-48</jats:sub>, <jats:italic>bla</jats:italic><jats:sub>NDM</jats:sub>, <jats:italic>bla</jats:italic><jats:sub>VIM</jats:sub> and <jats:italic>bla</jats:italic><jats:sub>KPC</jats:sub>, which account for 97% of the UK’s reported carbapenemase-producing Enterobacteriaceae.</jats:p>
AU - Rodriguez-Manzano,J
AU - Moniri,A
AU - Malpartida-Cardenas,K
AU - Dronavalli,J
AU - Davies,F
AU - Holmes,A
AU - Georgiou,P
DO - 10.1101/409912
PY - 2018///
TI - Simultaneous single-channel multiplex and quantification of carbapenem-resistant genes using multidimensional standard curves
UR - http://dx.doi.org/10.1101/409912
ER -