8 results found
Bignold R, Shammout B, Rowley JE, et al., 2022, Chemokine CXCL12 drives pericyte accumulation and airway remodeling in allergic airway disease, RESPIRATORY RESEARCH, Vol: 23
Ha KP, Clarke RS, Kim G-L, et al., 2020, Staphylococcal DNA repair Is required for infection, mBio, Vol: 11, ISSN: 2150-7511
To cause infection, Staphylococcus aureus must withstand damage caused by host immune defenses. However, the mechanisms by which staphylococcal DNA is damaged and repaired during infection are poorly understood. Using a panel of transposon mutants, we identified the rexBA operon as being important for the survival of Staphylococcus aureus in whole human blood. Mutants lacking rexB were also attenuated for virulence in murine models of both systemic and skin infections. We then demonstrated that RexAB is a member of the AddAB family of helicase/nuclease complexes responsible for initiating the repair of DNA double-strand breaks. Using a fluorescent reporter system, we were able to show that neutrophils cause staphylococcal DNA double-strand breaks through reactive oxygen species (ROS) generated by the respiratory burst, which are repaired by RexAB, leading to the induction of the mutagenic SOS response. We found that RexAB homologues in Enterococcus faecalis and Streptococcus gordonii also promoted the survival of these pathogens in human blood, suggesting that DNA double-strand break repair is required for Gram-positive bacteria to survive in host tissues. Together, these data demonstrate that DNA is a target of host immune cells, leading to double-strand breaks, and that the repair of this damage by an AddAB-family enzyme enables the survival of Gram-positive pathogens during infection.IMPORTANCE To cause infection, bacteria must survive attack by the host immune system. For many bacteria, including the major human pathogen Staphylococcus aureus, the greatest threat is posed by neutrophils. These immune cells ingest the invading organisms and try to kill them with a cocktail of chemicals that includes reactive oxygen species (ROS). The ability of S. aureus to survive this attack is crucial for the progression of infection. However, it was not clear how the ROS damaged S. aureus and how the bacterium repaired this damage. In this work, we show that ROS cause breaks
Johnson J, Rowley JE, 2018, Lung-Resident Pericytes Acquire Increased Migratory Capacity in a House Dust Mite-Driven Model of Asthma, International Conference of the American-Thoracic-Society, Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Johnson JR, Rowley JE, Bloxham R, et al., 2017, Lung-Resident Pericytes Acquire Myofibroblast Functions In A House Dust Mite-Driven Model Of Asthma, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Johnson JR, Folestad E, Rowley JE, et al., 2015, Pericytes contribute to airway remodeling in a mouse model of chronic allergic asthma, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 308, Pages: L658-L671, ISSN: 1040-0605
Wong S-P, Rowley JE, Redpath AN, et al., 2015, Pericytes, mesenchymal stem cells and their contributions to tissue repair, Pharmacology & Therapeutics, Vol: 151, Pages: 107-120, ISSN: 0163-7258
Regenerative medicine using mesenchymal stem cells for the purposes of tissue repair has garnered considerablepublic attention due to the potential of returning tissues and organs to a normal, healthy state after injury or damagehas occurred. To achieve this, progenitor cells such as pericytes and bone marrow-derived mesenchymalstem cells can be delivered exogenously, mobilised and recruited from within the body or transplanted in theform organs and tissues grown in the laboratory from stem cells. In this review, we summarise the recent evidencesupporting the use of endogenously mobilised stem cell populations to enhance tissue repair along withthe use of mesenchymal stem cells and pericytes in the development of engineered tissues. Finally, we concludewith an overview of currently available therapeutic options to manipulate endogenous stem cells to promotetissue repair.
Rowley JE, Johnson JR, 2014, Pericytes in Chronic Lung Disease, INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol: 164, Pages: 178-188, ISSN: 1018-2438
Rowley JE, Johnson JR, 2014, Pericytes Contribute To Airway Smooth Muscle Thickening And Comprise A Lung Resident Population Of Mesenchymal Stem Cells, Publisher: AMER THORACIC SOC, ISSN: 1073-449X
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