Imperial College London

ProfessorJohnSeddon

Faculty of Natural SciencesDepartment of Chemistry

Professor of Chemical Physics
 
 
 
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Contact

 

+44 (0)20 7594 5797j.seddon Website

 
 
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Location

 

207EMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@inproceedings{Ces:2016,
author = {Ces, O and Elani, Y and Karamdad, K and Friddin, MS and Barter, LMC and Bolognesi, G and Law, RV and Chan, CL and Brooks, NJ and Seddon, JM},
title = {Novel microfluidic technologies for the bottom-up construction of artificial cells},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - CPAPER
AB - © 2016 Institution of Engineering and Technology. All rights reserved. This talk will outline novel microfluidic strategies for biomembrane engineering that are capable of fabricating vesicles [1], droplet interface bilayer networks [2], multisomes [3] and artificial tissues [4] where parameters such as membrane asymmetry, membrane curvature, compartment connectivity and individual compartment contents can be controlled. Various bulk methods, such as extrusion, gentle hydration and electroformation, have been synonymous with the formation of lipid vesicles over recent years. However these strategies suffer from significant shortcomings associated with these processes including limited control of vesicle structural parameters such as size, lamellarity, membrane composition and internal contents. To address this technological bottleneck we have developed novel microfluidic platforms to form lipid vesicles in high-Throughput with full control over the composition of both the inner and outer leaflet of the membrane thereby enabling the manufacture of symmetric and asymmetric vesicles. This is achieved by manufacturing microfluidic channels with a step junction, produced by double-layer photolithography, which facilitates the transfer of a W/O emulsion across an oil-water phase boundary and the self-Assembly of a phospholipid bilayer. These platforms are being used to explore the role of asymmetry in biological systems [1] and study the engineering rules that regulate membrane mediated protein-protein interactions [5]. In addition, these technologies are enabling the construction of biological machines capable of acting as micro-reactors [6], environmental sensors and smart delivery vehicles [5] as well as complex multi-compartment artificial cells where the contents and connectivity of each compartment can be controlled. These compartments are separated by biological functional membranes that can facilitate transport between the compartments themselves and between
AU - Ces,O
AU - Elani,Y
AU - Karamdad,K
AU - Friddin,MS
AU - Barter,LMC
AU - Bolognesi,G
AU - Law,RV
AU - Chan,CL
AU - Brooks,NJ
AU - Seddon,JM
PY - 2016///
TI - Novel microfluidic technologies for the bottom-up construction of artificial cells
ER -