Imperial College London

Emeritus ProfessorJonathanStoye

Faculty of MedicineDepartment of Infectious Disease

Emeritus Professor of Endogenous Retroviruses
 
 
 
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Contact

 

j.stoye Website

 
 
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Location

 

Francis Crick InstituteThe Francis Crick Institute

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Summary

 

Publications

Citation

BibTex format

@article{Cottee:2019:10.1101/691972,
author = {Cottee, MA and Letham, SC and Young, GR and Stoye, JP and Taylor, IA},
doi = {10.1101/691972},
title = {Structure of <i>D. melanogaster</i> ARC1 reveals a repurposed molecule with characteristics of retroviral Gag},
url = {http://dx.doi.org/10.1101/691972},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - <jats:title>ABSTRACT</jats:title><jats:p>The tetrapod neuronal protein ARC and its <jats:italic>D. melanogaster</jats:italic> homologue, dARC1, have important but differing roles in neuronal development. Both are thought to originate through exaptation of ancient Ty3/Gypsy retrotransposon Gag genes, with their novel function relying on an original capacity for self-assembly and encapsidation of nucleic acids. Here, we present the crystal structure of dARC1 CA and examine the relationship between dARC1, mammalian ARC and the CA protein of circulating retroviruses. We show that whilst the overall architecture is highly related to that of orthoretroviral and spumaretroviral CA, there are significant deviations in both N- and C-terminal domains, potentially affecting recruitment of partner proteins and particle assembly. The degree of sequence and structural divergence suggests that Ty3/Gypsy Gag has been exapted on two separate occasions and that, although mammalian ARC and dARC1 share functional similarity, the structures have undergone different adaptations after appropriation into the tetrapod and insect genomes.</jats:p>
AU - Cottee,MA
AU - Letham,SC
AU - Young,GR
AU - Stoye,JP
AU - Taylor,IA
DO - 10.1101/691972
PY - 2019///
TI - Structure of <i>D. melanogaster</i> ARC1 reveals a repurposed molecule with characteristics of retroviral Gag
UR - http://dx.doi.org/10.1101/691972
ER -