66 results found
Duchemin A-L, Vignes H, Vermot J, 2019, Mechanically activated piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis, eLife, Vol: 8, Pages: 1-27, ISSN: 2050-084X
Mechanical forces are well known for modulating heart valve developmental programs. Yet, it is still unclear how genetic programs and mechanosensation interact during heart valve development. Here, we assessed the mechanosensitive pathways involved during zebrafish outflow tract (OFT) valve development in vivo. Our results show that the hippo effector Yap1, Klf2, and the Notch signaling pathway are all essential for OFT valve morphogenesis in response to mechanical forces, albeit active in different cell layers. Furthermore, we show that Piezo and TRP mechanosensitive channels are important factors modulating these pathways. In addition, live reporters reveal that Piezo controls Klf2 and Notch activity in the endothelium and Yap1 localization in the smooth muscle progenitors to coordinate OFT valve morphogenesis. Together, this work identifies a unique morphogenetic program during OFT valve formation and places Piezo as a central modulator of the cell response to forces in this process.
Duchemin A-L, Vignes H, Vermot J, et al., 2019, Mechanotransduction in cardiovascular morphogenesis and tissue engineering, CURRENT OPINION IN GENETICS & DEVELOPMENT, Vol: 57, Pages: 106-116, ISSN: 0959-437X
Andres-Delgado L, Ernst A, Galardi-Castilla M, et al., 2019, Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells, DEVELOPMENT, Vol: 146, ISSN: 0950-1991
Galvez-Santisteban M, Chen D, Zhang R, et al., 2019, Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming, ELIFE, Vol: 8, ISSN: 2050-084X
Ferreira RR, Pakula G, Klaeyle L, et al., 2018, Chiral Cilia orientation in the left-right organizer, Cell Reports, Vol: 25, Pages: 2008-2016.e4, ISSN: 2211-1247
Chirality is a property of asymmetry between an object and its mirror image. Most biomolecules and many cell types are chiral. In the left-right organizer (LRO), cilia-driven flows transfer such chirality to the body scale. However, the existence of cellular chirality within tissues remains unknown. Here, we investigate this question in Kupffer’s vesicle (KV), the zebrafish LRO. Quantitative live imaging reveals that cilia populating the KV display asymmetric orientation between the right and left sides, resulting in a chiral structure, which is different from the chiral cilia rotation. This KV chirality establishment is dynamic and depends on planar cell polarity. While its impact on left-right (LR) symmetry breaking remains unclear, we show that this asymmetry does not depend on the LR signaling pathway or flow. This work identifies a different type of tissue asymmetry and sheds light on chirality genesis in developing tissues.
Campinho P, Lamperti P, Boselli F, et al., 2018, Three-dimensional microscopy and image analysis methodology for mapping and quantification of nuclear positions in tissues with approximate cylindrical geometry, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 373, ISSN: 0962-8436
Fukui H, Miyazaki T, Chow RW-Y, et al., 2018, Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish, ELIFE, Vol: 7, ISSN: 2050-084X
Chow RW-Y, Lamperti P, Steed E, et al., 2018, Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X
Boselli F, Steed E, Freund JB, et al., 2017, Anisotropic shear stress patterns predict the orientation of convergent tissue movements in the embryonic heart, DEVELOPMENT, Vol: 144, Pages: 4322-4327, ISSN: 0950-1991
Goddard LM, Duchemin A-L, Ramalingan H, et al., 2017, Hemodynamic Forces Sculpt Developing Heart Valves through a KLF2-WNT9B Paracrine Signaling Axis, DEVELOPMENTAL CELL, Vol: 43, Pages: 274-+, ISSN: 1534-5807
Bouchaala R, Anton N, Anton H, et al., 2017, Light-triggered release from dye-loaded fluorescent lipid nanocarriers in vitro and in vivo, COLLOIDS AND SURFACES B-BIOINTERFACES, Vol: 156, Pages: 414-421, ISSN: 0927-7765
Ferreira RR, Vilfan A, Juelicher F, et al., 2017, Physical limits of flow sensing in the left right organizer, ELIFE, Vol: 6, ISSN: 2050-084X
Ferreira RR, Vermot J, 2017, The balancing roles of mechanical forces during left-right patterning and asymmetric morphogenesis, MECHANISMS OF DEVELOPMENT, Vol: 144, Pages: 71-80, ISSN: 0925-4773
Chow RW-Y, Vermot J, 2017, The rise of photoresponsive protein technologies applications in vivo: a spotlight on zebrafish developmental and cell biology., F1000Res, Vol: 6, ISSN: 2046-1402
The zebrafish ( Danio rerio) is a powerful vertebrate model to study cellular and developmental processes in vivo. The optical clarity and their amenability to genetic manipulation make zebrafish a model of choice when it comes to applying optical techniques involving genetically encoded photoresponsive protein technologies. In recent years, a number of fluorescent protein and optogenetic technologies have emerged that allow new ways to visualize, quantify, and perturb developmental dynamics. Here, we explain the principles of these new tools and describe some of their representative applications in zebrafish.
Jacob L, Sawma P, Garnier N, et al., 2016, Inhibition of PlexA1-mediated brain tumor growth and tumor-associated angiogenesis using a transmembrane domain targeting peptide, ONCOTARGET, Vol: 7, Pages: 57851-57865
Steed E, Boselli F, Vermot J, 2016, Hemodynamics driven cardiac valve morphogenesis, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, Vol: 1863, Pages: 1760-1766, ISSN: 0167-4889
Steed E, Faggianelli N, Roth S, et al., 2016, klf2a couples mechanotransduction and zebrafish valve morphogenesis through fibronectin synthesis, Nature Communications, Vol: 7, ISSN: 2041-1723
The heartbeat and blood flow signal to endocardial cell progenitors through mechanosensitive proteins that modulate the genetic program controlling heart valve morphogenesis. To date, the mechanism by which mechanical forces coordinate tissue morphogenesis is poorly understood. Here we use high-resolution imaging to uncover the coordinated cell behaviours leading to heart valve formation. We find that heart valves originate from progenitors located in the ventricle and atrium that generate the valve leaflets through a coordinated set of endocardial tissue movements. Gene profiling analyses and live imaging reveal that this reorganization is dependent on extracellular matrix proteins, in particular on the expression of fibronectin1b. We show that blood flow and klf2a, a major endocardial flow-responsive gene, control these cell behaviours and fibronectin1b synthesis. Our results uncover a unique multicellular layering process leading to leaflet formation and demonstrate that endocardial mechanotransduction and valve morphogenesis are coupled via cellular rearrangements mediated by fibronectin synthesis.
Boselli F, Vermot J, 2016, Live imaging and modeling for shear stress quantification in the embryonic zebrafish heart, METHODS, Vol: 94, Pages: 129-134, ISSN: 1046-2023
Boselli F, Freund JB, Vermot J, 2015, Blood flow mechanics in cardiovascular development, CELLULAR AND MOLECULAR LIFE SCIENCES, Vol: 72, Pages: 2545-2559, ISSN: 1420-682X
Ramspacher C, Steed E, Boselli F, et al., 2015, Developmental Alterations in Heart Biomechanics and Skeletal Muscle Function in Desmin Mutants Suggest an Early Pathological Root for Desminopathies, CELL REPORTS, Vol: 11, Pages: 1564-1576, ISSN: 2211-1247
Hnia K, Ramspacher C, Vermot J, et al., 2015, Desmin in muscle and associated diseases: beyond the structural function, CELL AND TISSUE RESEARCH, Vol: 360, Pages: 591-608, ISSN: 0302-766X
Heckel E, Boselli F, Roth S, et al., 2015, Oscillatory Flow Modulates Mechanosensitive klf2a Expression through trpv4 and trpp2 during Heart Valve Development, CURRENT BIOLOGY, Vol: 25, Pages: 1354-1361, ISSN: 0960-9822
Renz M, Otten C, Faurobert E, et al., 2015, Regulation of beta 1 Integrin-Klf2-Mediated Angiogenesis by CCM Proteins, DEVELOPMENTAL CELL, Vol: 32, Pages: 181-190, ISSN: 1534-5807
Mahou P, Vermot J, Beaurepaire E, et al., 2015, Multiphoton light-sheet microscopy using wavelength mixing: fast multicolor imaging of the beating zebrafish heart with low photobleaching, Conference on Multiphoton Microscopy in the Biomedical Sciences XV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Boselli F, Goetz JG, Charvin G, et al., 2015, A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in vivo, METHODS IN CILIA & FLAGELLA, Vol: 127, Pages: 161-173, ISSN: 0091-679X
Goetz JG, Monduc F, Schwab Y, et al., 2015, Using correlative light and electron microscopy to study zebrafish vascular morphogenesis., Methods Mol Biol, Vol: 1189, Pages: 31-46
Live imaging is extremely useful to characterize the dynamics of cellular events in vivo, yet it is limited in terms of spatial resolution. Correlative light and electron microscopy (CLEM) allows combining live confocal microscopy with electron microscopy (EM) for the characterization of biological samples at high temporal and spatial resolution. Here we describe a protocol allowing extracting endothelial cell ultrastructure after having imaged the same cell in its in vivo context through live confocal imaging during zebrafish embryonic development.
Renz M, Otten C, Rudolph F, et al., 2014, Exiting angiogenesis requires CCM proteins to attenuate proangiogenic Klf2 activity, Publisher: SPRINGER, Pages: 719-719, ISSN: 0969-6970
Kilin VN, Anton H, Anton N, et al., 2014, Counterion-enhanced cyanine dye loading into lipid nano-droplets for single-particle tracking in zebrafish, BIOMATERIALS, Vol: 35, Pages: 4950-4957, ISSN: 0142-9612
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