Imperial College London
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DrJulienVermot

Faculty of Engineering, Department of Bioengineering

Reader in Development Bio-mechanochemical Signalling
 
 
 
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Contact

 

+44 (0)20 7594 8820j.vermot Website

 
 
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Location

 

B213EBessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Steib:2023:10.1016/j.xpro.2023.102257,
author = {Steib, E and Vagena-Pantoula, C and Vermot, J},
doi = {10.1016/j.xpro.2023.102257},
journal = {STAR Protocols},
title = {TissUExM protocol for ultrastructure expansion microscopy of zebrafish larvae and mouse embryos},
url = {http://dx.doi.org/10.1016/j.xpro.2023.102257},
volume = {4},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Expansion microscopy of millimeter-large mechanically heterogeneous tissues, such as whole vertebrate embryos, has been limited, particularly when combined with post-expansion immunofluorescence. Here, we present a protocol to perform ultrastructure expansion microscopy of whole vertebrate embryos, optimized to perform post-expansion labeling. We describe steps for embedding and denaturing zebrafish larvae or mouse embryos. We then detail procedures for hydrogel handling and mounting. This protocol is particularly well suited for super-resolution imaging of macromolecular protein complexes in situ but does not preserve lipids. For complete details on the use and execution of this protocol, please refer to Steib et al.1.
AU  - Steib,E
AU  - Vagena-Pantoula,C
AU  - Vermot,J
DO  - 10.1016/j.xpro.2023.102257
PY  - 2023///
SN  - 2666-1667
TI  - TissUExM protocol for ultrastructure expansion microscopy of zebrafish larvae and mouse embryos
T2  - STAR Protocols
UR  - http://dx.doi.org/10.1016/j.xpro.2023.102257
UR  - https://www.ncbi.nlm.nih.gov/pubmed/37119141
UR  - http://hdl.handle.net/10044/1/104059
VL  - 4
ER  -
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