Imperial College London
Portrait Picture

Professor Jake Baum

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 5420jake.baum Website

 
 
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Location

 

c/o Baum labSir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haase:2021:10.1016/j.molbiopara.2021.111374,
author = {Haase, S and miller, D and Cherkaoui, D and Jordan, S and Gulbis, J and Baum, J},
doi = {10.1016/j.molbiopara.2021.111374},
journal = {Molecular and Biochemical Parasitology},
pages = {1--15},
title = {Identification and characterisation of a phospholipid scramblase in the malaria parasite Plasmodium falciparum},
url = {http://dx.doi.org/10.1016/j.molbiopara.2021.111374},
volume = {243},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Recent studies highlight the emerging role of lipids as important messengers in malaria parasite biology. In an attempt to identify interacting proteins and regulators of these dynamic and versatile molecules, we hypothesised the involvement of phospholipid translocases and their substrates in the infection of the host erythrocyte by the malaria parasite Plasmodium spp. Here, using a data base searching approach of the Plasmodium Genomics Resources (www.plasmodb.org), we have identified a putative phospholipid (PL) scramblase in P. falciparum (PfPLSCR) that is conserved across the genus and in closely related unicellular algae. By reconstituting recombinant PfPLSCR into liposomes, we demonstrate metal ion dependent PL translocase activity and substrate preference, confirming PfPLSCR as a bona fide scramblase. We show that PfPLSCR is expressed during asexual and sexual parasite development, localising to different membranous compartments of the parasite throughout the intra-erythrocytic life cycle. Two different gene knockout approaches, however, suggest that PfPLSCR is not essential for erythrocyte invasion and asexual parasite development, pointing towards a possible role in other stages of the parasite life cycle.
AU  - Haase,S
AU  - miller,D
AU  - Cherkaoui,D
AU  - Jordan,S
AU  - Gulbis,J
AU  - Baum,J
DO  - 10.1016/j.molbiopara.2021.111374
EP  - 15
PY  - 2021///
SN  - 0166-6851
SP  - 1
TI  - Identification and characterisation of a phospholipid scramblase in the malaria parasite Plasmodium falciparum
T2  - Molecular and Biochemical Parasitology
UR  - http://dx.doi.org/10.1016/j.molbiopara.2021.111374
UR  - https://www.sciencedirect.com/science/article/pii/S0166685121000256?via%3Dihub
UR  - http://hdl.handle.net/10044/1/89676
VL  - 243
ER  -
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