Imperial College London
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Professor Jake Baum

Faculty of Natural Sciences, Department of Life Sciences

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 5420jake.baum Website

 
 
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Location

 

c/o Baum labSir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Malpartida-Cardenas:2022:10.1101/2022.07.28.501845,
author = {Malpartida-Cardenas, K and Baum, J and Cunnington, A and Georgiou, P and Rodriguez-Manzano, J},
doi = {10.1101/2022.07.28.501845},
title = {Electricity-free nucleic acid extraction method from dried blood spots on filter paper for point-of-care diagnostics},
url = {http://dx.doi.org/10.1101/2022.07.28.501845},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Nucleic acid extraction is a crucial step for molecular biology applications, being a determinant for any diagnostic test procedure. Dried blood spots (DBS) have been used for decades for serology, drug monitoring, environmental investigations, and molecular studies. Nevertheless, nucleic acid extraction from DBS remains one of the main challenges to translate them to the point-of-care (POC).</jats:p></jats:sec><jats:sec><jats:title>Method</jats:title><jats:p>We have developed a fast nucleic acid extraction (NAE) method from DBS which is electricity-free and relies on cellulose filter papers (DBSFP). The performance of NAE was assessed with loop-mediated isothermal amplification (LAMP), targeting the human reference gene beta-actin. The developed method was evaluated against FTA cards and magnetic bead-based purification, using time-to-positive (min) for comparative analysis. We optimised and validated the developed method for elution (<jats:italic>eluted disk</jats:italic>) and disk directly in the reaction (<jats:italic>in-situ disk)</jats:italic>, RNA and DNA detection, and whole blood stored in anticoagulants (K<jats:sub>2</jats:sub>EDTA and lithium heparin). Furthermore, the compatibility of DBSFP with colourimetric detection was studied to show the transferability to the POC.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The proposed DBSFP is based on grade 3 filter paper pre-treated with 8% (v/v) igepal surfactant, 1 min washing step with PBS 1X and elution in TE 1X buffer after 5 min incubation at room temperature, enabling NAE under 7 min. Obtained results were comparable to gold standard methods across tested matrices, targets and experimental conditions, demonstrating the versatility of the methodology. Las
AU  - Malpartida-Cardenas,K
AU  - Baum,J
AU  - Cunnington,A
AU  - Georgiou,P
AU  - Rodriguez-Manzano,J
DO  - 10.1101/2022.07.28.501845
PY  - 2022///
TI  - Electricity-free nucleic acid extraction method from dried blood spots on filter paper for point-of-care diagnostics
UR  - http://dx.doi.org/10.1101/2022.07.28.501845
ER  -
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