Publications
102 results found
McGinty J, Stuckey D, Laine R, et al., 2010, Time-domain fluorescence lifetime optical projection tomography
We present a platform for measuring the fluorescence lifetime distribution in mesoscopic samples (~0.1-1cm) based on optical projection tomography and time-gated imaging. This is applied to optically cleared embryos expressing a calcium sensing FRET probe. © OSA / BIOMED/DH 2010.
Soloviev VY, McGinty J, Tahir KB, et al., 2010, Tomographic imaging of Fluorescence Resonance Energy Transfer in highly light scattering media, Conference on Biomedical Applications of Light Scattering IV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 1
McGinty J, Stuckey DW, Tahir KB, et al., 2010, <i>tomo</i>FLIM - fluorescence lifetime projection tomography, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XVII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
McGinty J, Soloviev VY, Tahir KB, et al., 2009, Three-dimensional imaging of Forster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging, OPT LETT, Vol: 34, Pages: 2772-2774, ISSN: 0146-9592
We report a three-dimensional time-resolved tomographic imaging technique for localizing protein-protein interaction and protein conformational changes in turbid media based on Forster resonant energy-transfer read out using fluorescence lifetime. This application of "tomoFRET" employs an inverse scattering algorithm utilizing the diffusion approximation to the radiative-transfer equation applied to a large tomographic data set of time-gated images. The approach is demonstrated by imaging a highly scattering cylindrical phantom within which are two thin wells containing cytosol preparations of HEK293 cells expressing TN-L15, a cytosolic genetically encoded calcium Forster resonant energy-transfer sensor. A 10 mM calcium chloride solution was added to one of the wells, inducing a protein conformation change upon binding to TN-L15, resulting in Forster resonant energy transfer and a corresponding decrease in the donor fluorescence lifetime. We successfully reconstruct spatially resolved maps of the resulting fluorescence lifetime distribution as well as of the quantum efficiency, absorption, and scattering coefficients. (C) 2009 Optical Society of America
McGinty J, Requejo-Isidro J, Munro I, et al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727
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- Citations: 19
McGinty J, Dunsby C, Auksorius E, et al., 2009, Chapter 4 Multidimensional fluorescence imaging
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- Citations: 3
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.
Talbot C, McGinty J, McGhee E, et al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy
We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.
Talbot CB, McGinty J, Grant DM, et al., 2008, High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 514-521, ISSN: 1864-063X
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- Citations: 40
McGinty J, Tahir KR, Laine R, et al., 2008, Fluorescence lifetime optical projection tomography, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 390-394, ISSN: 1864-063X
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- Citations: 39
Galletly NP, McGinty J, Dunsby C, et al., 2008, Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin, BRITISH JOURNAL OF DERMATOLOGY, Vol: 159, Pages: 152-161, ISSN: 0007-0963
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- Citations: 104
McGinty J, Tahir KB, Soloviev VY, et al., 2008, Time-domain fluorescence lifetime tomography
We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, OPTICS EXPRESS, Vol: 15, Pages: 15656-15673, ISSN: 1094-4087
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- Citations: 65
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, Optics Express, Vol: 15, Pages: 16656-16673
We present a time domain optically sectioned fluorescence lifetime imaging (FLIM) microscope developed for high-speed live cell imaging. This single photon excited system combines wide field parallel pixel detection with confocal sectioning utilizing spinning Nipkow disc microscopy. It can acquire fluorescence lifetime images of live cells at up to 10 frames per second (fps), permitting high-speed FLIM of cell dynamics and protein interactions with potential for high throughput cell imaging and screening applications. We demonstrate the application of this FLIM microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET. © 2007 Optical Society of America.
Soloviev VY, Tahir KB, McGinty J, et al., 2007, Fluorescence lifetime imaging by using time-gated data acquisition, APPLIED OPTICS, Vol: 46, Pages: 7384-7391, ISSN: 1559-128X
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- Citations: 49
Soloviev VY, McGinty J, Tahir KB, et al., 2007, Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence, OPTICS LETTERS, Vol: 32, Pages: 2034-2036, ISSN: 0146-9592
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- Citations: 23
Soloviev VY, Tahir KB, McGinty J, et al., 2007, Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data, European Conference on Biomedical Optics, Publisher: SPIE
De Beule P, Owen DM, Manning HB, et al., 2007, Rapid hyperspectral fluorescence lifetime imaging, MICROSCOPY RESEARCH AND TECHNIQUE, Vol: 70, Pages: 481-484, ISSN: 1059-910X
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- Citations: 44
Soloviev VY, Tahir KB, McGinty J, et al., 2007, Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data, Conference on Diffuse Optical Imaging of Tissue, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
Owen DM, Manning HB, de Beule P, et al., 2007, Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 2
Owen D M, Manning H B, de Beule P, et al., 2007, Rapid hyperspectral fluorescence lifetime imaging, IMAGING, MANIPULATION AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES V, BIOS, Publisher: SPIE
Galletly N, McGinty J, Munro I, et al., 2006, Fluorescence lifetime imaging of liver cancer, Digestive Disease Week/107th Annual Meeting of the American-Gastroenterological-Association, Publisher: W B SAUNDERS CO-ELSEVIER INC, Pages: A791-A791, ISSN: 0016-5085
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- Citations: 1
Lekadir, Karim, Elson, et al., 2006, Tissue Characterization using Dimensionality Reduction and Fluorescence Imaging, Medical Image Computing and Computer Assisted Intervention, Publisher: Medical Image Computing and Computer Assisted Intervention
Lanigan PMP, Dunsby C, McGinty J, et al., 2006, An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy
We demonstrate that spectral selection from a supercontinuum generated in a microstructured fibre can provide a continuously electronically tunable ultrafast spatially coherent source for confocal microscopy and both scanning and wide field fluorescence lifetime imaging. © 2005 Optical Society of America.
Lekadir K, Elson DS, Requejo-Isidro J, et al., 2006, Tissue characterization using dimensionality reduction and fluorescence imaging, MEDICAL IMAGE COMPUTING AND COMPUTER-ASSISTED INTERVENTION - MICCAI 2006, PT 2, Vol: 4191, Pages: 586-593, ISSN: 0302-9743
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- Citations: 13
Munro I, McGinty J, Galletly N, et al., 2005, Toward the clinical application of time-domain fluorescence lifetime imaging, JOURNAL OF BIOMEDICAL OPTICS, Vol: 10, ISSN: 1083-3668
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- Citations: 54
Benninger RKP, Hofmann O, McGinty J, et al., 2005, Time-resolved fluorescence imaging of solvent interactions in microfluidic devices, OPTICS EXPRESS, Vol: 13, Pages: 6275-6285, ISSN: 1094-4087
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- Citations: 46
Benninger RKP, McGinty J, Hofmann O, et al., 2005, Application of multi-dimensional fluorescence imaging to microfluidic devices, European Conference on Lasers and Electro-Optics
Benninger RKP, Hofmann O, McGinty J, et al., 2005, 3D high-speed time-resolved fluorescence imaging of solvent interactions in microfluidic devices, Pages: 16-18
This paper reports on the application of novel wide-field time-resolved fluorescence imaging methods to monitor solvent interactions and mixing in microfluidic devices. Time-resolved fluorescence anisotropy imaging (TR-FAIM) is employed for 3D viscosity mapping with sub-micron resolution. To image dynamic fluid mixing in real-time, we demonstrate high-speed fluorescence lifetime imaging (FLIM) at video acquisition rates. Copyright © 2005 by the Transducer Research Foundation, Inc.
Dunsby C, Lanigan PMP, McGinty J, et al., 2005, An electronically tunable ultrafast laser source applied to fluorescence imaging and microscopy including fluorescence lifetime imaging
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