Imperial College London
Portrait Picture

Professor James Seddon

Faculty of Medicine, Department of Infectious Disease

Professor of Global Child Health
 
 
 
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Contact

 

+44 (0)20 7594 3179james.seddon

 
 
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Location

 

235Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Manyelo:2021:10.1371/journal.pone.0250944,
author = {Manyelo, CM and Chegou, NN and Seddon, JA and Snyders, CI and Mutavhatsindi, H and Manngo, PM and Walzl, G and Stanley, K and Solomons, RS},
doi = {10.1371/journal.pone.0250944},
journal = {PLoS One},
pages = {1--18},
title = {Serum and cerebrospinal fluid host proteins indicate stroke in children with tuberculous meningitis},
url = {http://dx.doi.org/10.1371/journal.pone.0250944},
volume = {16},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - IntroductionStroke is a common complication in children with tuberculous meningitis (TBM). Host proteins may give us insight into the mechanisms of stroke in TBM and serve as biomarkers for detection of stroke, however, they have not been widely explored. In this study, we compared the concentrations of cerebrospinal fluid (CSF) and serum proteins between children who had TBM-related stroke and children with TBM without stroke.MethodsWe collected CSF and serum from 47 children consecutively admitted to the Tygerberg Academic Hospital in Cape Town, South Africa between November 2016, and November 2017, on suspicion of having TBM. A multiplex platform was used to measure the concentrations of 69 host proteins in CSF and serum from all study participants.ResultsAfter classification of study participants, 23 (48.9%) out of the 47 study participants were diagnosed with TBM, of which 14 (60.9%) demonstrated radiological arterial ischemic infarction. The levels of lipocalin-2, sRAGE, IP-10/ CXCL10, sVCAM-1, MMP-1, and PDGF-AA in CSF samples and the levels of D-dimer, ADAMTS13, SAA, ferritin, MCP-1/ CCL2, GDF-15 and IL-13 in serum samples were statistically different between children who had TBM-related stroke and children with TBM without stroke. After correcting for multiple testing, only the levels of sVCAM-1, MMP-1, sRAGE, and IP-10/ CXCL10 in CSF were statistically different between the two groups. CSF and serum protein biosignatures indicated stroke in children diagnosed with TBM with up to 100% sensitivity and 88.9% specificity.ConclusionSerum and CSF proteins may serve as biomarkers for identifying individuals with stroke amongst children diagnosed with TBM at admission and may guide us to understand the biology of stroke in TBM. This was a pilot study, and thus further investigations in larger studies are needed.
AU  - Manyelo,CM
AU  - Chegou,NN
AU  - Seddon,JA
AU  - Snyders,CI
AU  - Mutavhatsindi,H
AU  - Manngo,PM
AU  - Walzl,G
AU  - Stanley,K
AU  - Solomons,RS
DO  - 10.1371/journal.pone.0250944
EP  - 18
PY  - 2021///
SN  - 1932-6203
SP  - 1
TI  - Serum and cerebrospinal fluid host proteins indicate stroke in children with tuberculous meningitis
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0250944
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000664607000054&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250944
UR  - http://hdl.handle.net/10044/1/94017
VL  - 16
ER  -
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