Imperial College London
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Professor James Seddon

Faculty of Medicine, Department of Infectious Disease

Professor of Global Child Health
 
 
 
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Contact

 

+44 (0)20 7594 3179james.seddon

 
 
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Location

 

235Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kaforou:2022:10.1111/imr.13116,
author = {Kaforou, M and Broderick, C and Vito, O and Levin, M and Scriba, T and Seddon, J},
doi = {10.1111/imr.13116},
journal = {Immunological Reviews},
title = {Transcriptomics for child and adolescent tuberculosis},
url = {http://dx.doi.org/10.1111/imr.13116},
volume = {309},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tuberculosis (TB) in humans is caused by Mycobacterium tuberculosis (Mtb). It is estimated that 70 million children (<15 years) are currently infected with Mtb, with 1.2 million each year progressing to disease. Of these, a quarter die. The risk of progression from Mtb infection to disease and from disease to death is dependent on multiple pathogen and host factors. Age is a central component in all these transitions. The natural history of TB in children and adolescents is different to adults, leading to unique challenges in the development of diagnostics, therapeutics, and vaccines. The quantification of RNA transcripts, encoded in the genome in specific cells or in the peripheral blood, using high-throughput methods, such as microarray analysis or RNA sequencing, are emerging technologies. RNA sequencing can shed light into the host immune response to Mtb during infection and disease, as well as understanding treatment response, disease severity and vaccination, in a global hypothesis-free manner. Additionally, gene expression profiling can be used for biomarker discovery, to diagnose disease, predict future disease progression and to monitor response to treatment. Here, we review the role of transcriptomics in children and adolescents, focussed mainly on work done in blood, to understand disease biology and to discriminate disease states to assist clinical decision-making. In recent years, studies with a specific paediatric and adolescent focus have identified blood gene expression markers with diagnostic or prognostic potential that meet or exceed the current sensitivity and specificity targets for diagnostic tools. Diagnostic and prognostic gene expression signatures identified through high-throughput methods are currently being translated into diagnostic tests.
AU  - Kaforou,M
AU  - Broderick,C
AU  - Vito,O
AU  - Levin,M
AU  - Scriba,T
AU  - Seddon,J
DO  - 10.1111/imr.13116
PY  - 2022///
SN  - 0105-2896
TI  - Transcriptomics for child and adolescent tuberculosis
T2  - Immunological Reviews
UR  - http://dx.doi.org/10.1111/imr.13116
UR  - https://onlinelibrary.wiley.com/doi/10.1111/imr.13116
UR  - http://hdl.handle.net/10044/1/98197
VL  - 309
ER  -
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