Imperial College London
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Professor Jerry Heng

Faculty of Engineering, Department of Chemical Engineering

Professor in Particle Technology
 
 
 
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Contact

 

+44 (0)20 7594 0784jerry.heng

 
 
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Location

 

208ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chen:2021:10.1016/j.seppur.2021.118358,
author = {Chen, W and Li, X and Guo, M and Link, FJ and Ramli, SS and Ouyang, J and Rosbottom, I and Heng, JYY},
doi = {10.1016/j.seppur.2021.118358},
journal = {Separation and Purification Technology},
pages = {1--15},
title = {Biopurification of monoclonal antibody (mAb) through crystallisation},
url = {http://dx.doi.org/10.1016/j.seppur.2021.118358},
volume = {263},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Therapeutics based on monoclonal antibody (mAb) represent the most advanced biopharmaceuticals, being able to treat a wide range of challenging diseases such as cancers and arthritis. As the scale of mAb production steadily increases with the demand for mAb-based therapeutics, the downstream biopurification continues to experience significant bottleneck due to the throughput limited nature of the current purification technology. Over the last decades, significant advances have been made in protein (and especially mAb) crystallisation as an alternative biopurification technology that offers high product stability and purity as well as scalability. This review starts with the discussion of general physicochemical properties of mAb before moving on to the in-depth discussion of the distinct phase behaviour of mAb in comparison with conventional globular proteins such as lysozyme. The final part of this review presents a summary of successful demonstrations of crystallisation scale-ups of mAb and discusses the critical factors (i.e. mixing and temperature control) to be considered.
AU  - Chen,W
AU  - Li,X
AU  - Guo,M
AU  - Link,FJ
AU  - Ramli,SS
AU  - Ouyang,J
AU  - Rosbottom,I
AU  - Heng,JYY
DO  - 10.1016/j.seppur.2021.118358
EP  - 15
PY  - 2021///
SN  - 1383-5866
SP  - 1
TI  - Biopurification of monoclonal antibody (mAb) through crystallisation
T2  - Separation and Purification Technology
UR  - http://dx.doi.org/10.1016/j.seppur.2021.118358
UR  - https://www.sciencedirect.com/science/article/pii/S1383586621000605?via%3Dihub
UR  - http://hdl.handle.net/10044/1/87003
VL  - 263
ER  -
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