Imperial College London
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DrJiaGuo

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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Contact

 

jia.guo

 
 
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Location

 

Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tang:2017:10.1016/j.vaccine.2017.03.062,
author = {Tang, X and Guo, J and Cheng, L and Sun, C and Liu, L and Zuo, T and Wang, H and Chen, L and Zhang, L and Chen, Z},
doi = {10.1016/j.vaccine.2017.03.062},
journal = {Vaccine},
pages = {2582--2591},
title = {Single N277A substitution in C2 of simian immunodeficiency virus envelope influences vaccine-elicited CD4i neutralizing and anti-V2 antibody responses},
url = {http://dx.doi.org/10.1016/j.vaccine.2017.03.062},
volume = {35},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - An effective HIV vaccine remains elusive, and immunogens capable of eliciting protective host humoral immunity have not yet been identified. Although HIV/SIV infections result in the abundant production of CD4-induced (CD4i) antibodies (Abs), these Abs are not protective due to steric restrictions following gp120 binding to CD4 on target cells. Here we report that both DNA- and vaccinia-based vaccines encoding SIVmac239 gp160 readily elicited high levels of CD4i Abs in experimental animals. We identified a highly conserved N-linked glycosylation site N277 in the C2 region which strongly affected the immunogenicity of the CD4i Ab domain. Moreover, a single N277A substitution significantly enhanced the immunogenicity of the V2 domain yielding higher titers and frequency of anti-V2 Ab responses as determined by ELISA and yeast antigen display mapping, respectively. Importantly, immune sera elicited by the N277A-mutated gp160 exhibited elevated antibody-dependent cellular cytotoxicity (ADCC) activity. ADCC activity correlated positively with the anti-V2 Ab titer yet, inversely with CD4i Ab titer. Thus, we identified a determinant of the CD4i domain that might affect vaccine-elicited anti-V2 Ab and ADCC responses to SIVmac239. Our findings may have implications for design of immunogens to direct B cell recognition in the development of an Ab-based HIV vaccine.
AU  - Tang,X
AU  - Guo,J
AU  - Cheng,L
AU  - Sun,C
AU  - Liu,L
AU  - Zuo,T
AU  - Wang,H
AU  - Chen,L
AU  - Zhang,L
AU  - Chen,Z
DO  - 10.1016/j.vaccine.2017.03.062
EP  - 2591
PY  - 2017///
SN  - 0264-410X
SP  - 2582
TI  - Single N277A substitution in C2 of simian immunodeficiency virus envelope influences vaccine-elicited CD4i neutralizing and anti-V2 antibody responses
T2  - Vaccine
UR  - http://dx.doi.org/10.1016/j.vaccine.2017.03.062
UR  - http://hdl.handle.net/10044/1/48766
VL  - 35
ER  -
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