Imperial College London
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Dr. Jia Li

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Reader in Biological Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3230jia.li

 
 
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Location

 

10.N2ACommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vonhof:2020:10.1021/acs.analchem.0c02377,
author = {Vonhof, EV and Piotto, M and Holmes, E and Lindon, JC and Nicholson, JK and Li, JV},
doi = {10.1021/acs.analchem.0c02377},
journal = {Analytical Chemistry},
pages = {11516--11519},
title = {Improved spatial resolution of metabolites in tissue biopsies using high-resolution magic-angle-spinning slice localization NMR spectroscopy.},
url = {http://dx.doi.org/10.1021/acs.analchem.0c02377},
volume = {92},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - High-resolution magic-angle-spinning 1H NMR spectroscopy (HR-MAS NMR) is a well-established technique for assessing the biochemical composition of intact tissue samples. In this study, we utilized a method based on HR-MAS NMR spectroscopy with slice localization (SLS) to achieve spatial resolution of metabolites. The obtained 7 slice spectra from each of the model samples (i.e., chicken thigh muscle with skin and murine renal biopsy including medulla (M) and cortex (C)) showed distinct metabolite compositions. Furthermore, we analyzed previously acquired 1H HR-MAS NMR spectra of separated cortex and medulla samples using multivariate statistical methods. Concentrations of glycerophosphocholine (GPC) were found to be significantly higher in the renal medulla compared to the cortex. Using GPC as a biomarker, we identified the tissue slices that were predominantly the cortex or medulla. This study demonstrates that HR-MAS SLS combined with multivariate statistics has the potential for identifying tissue heterogeneity and detailed biochemical characterization of complex tissue samples.
AU  - Vonhof,EV
AU  - Piotto,M
AU  - Holmes,E
AU  - Lindon,JC
AU  - Nicholson,JK
AU  - Li,JV
DO  - 10.1021/acs.analchem.0c02377
EP  - 11519
PY  - 2020///
SN  - 0003-2700
SP  - 11516
TI  - Improved spatial resolution of metabolites in tissue biopsies using high-resolution magic-angle-spinning slice localization NMR spectroscopy.
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.0c02377
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32815363
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c02377
UR  - http://hdl.handle.net/10044/1/82941
VL  - 92
ER  -
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