Imperial College London
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Dr John S Tregoning

Faculty of Medicine, Department of Infectious Disease

Professor in Vaccine Immunology
 
 
 
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Contact

 

john.tregoning Website

 
 
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Location

 

456 (Shattock Group)Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haeusler:2022:cei/uxac066,
author = {Haeusler, IL and Daniel, O and Isitt, C and Watts, R and Cantrell, L and Feng, S and Cochet, M and Salloum, M and Ikram, S and Hayter, E and Lim, S and Hall, T and Athaide, S and Cosgrove, CA and Tregoning, JS and Le, Doare K},
doi = {cei/uxac066},
journal = {Clinical and Experimental Immunology},
pages = {188--200},
title = {Group B Streptococcus (GBS) colonisation is dynamic over time, whilst GBS capsular polysaccharides-specific antibody remains stable},
url = {http://dx.doi.org/10.1093/cei/uxac066},
volume = {209},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Group B Streptococcus (GBS) is a leading cause of adverse pregnancy outcomes due to invasive infection. This study investigated longitudinal variation in GBS rectovaginal colonisation, serum and vaginal GBS capsular polysaccharide (CPS)-specific antibody levels. Non-pregnant women were recruited in the UK, and were sampled every two weeks over a 12-week period. GBS isolates were taken from recto-vaginal swabs and serotyped by polymerase chain reaction. Serum and vaginal immunoglobulin G (IgG) and nasal immunoglobulin A (IgA) specific to CPS were measured by Luminex, and total IgG/A by ELISA. 70 women were enrolled, of median age 26. Out of the 66 participants who completed at least three visits: 14/47 (29.8%) women that were GBS negative at screening became positive in follow up visits and 16/19 (84.2%) women who were GBS positive at screening became negative. There was 50% probability of becoming negative 36 days after the first positive swab. The rate of detectable GBS carriage fluctuated over time, although serum, vaginal and nasal CPS-specific antibody levels remained constant. Levels of CPS-specific antibodies were higher in the serum of individuals colonised with GBS than in non-colonised, but similar in the vaginal and nasal mucosa. We found correlations between antibody levels in serum and the vaginal and nasal mucosa. Our study demonstrates the feasibility of elution methods to retrieve vaginal and nasal antibodies, and the optimisation of immunoassays to measure GBS-CPS specific antibodies. The difference between the dynamics of colonisation and antibody response is interesting and further investigation is required for vaccine development.
AU  - Haeusler,IL
AU  - Daniel,O
AU  - Isitt,C
AU  - Watts,R
AU  - Cantrell,L
AU  - Feng,S
AU  - Cochet,M
AU  - Salloum,M
AU  - Ikram,S
AU  - Hayter,E
AU  - Lim,S
AU  - Hall,T
AU  - Athaide,S
AU  - Cosgrove,CA
AU  - Tregoning,JS
AU  - Le,Doare K
DO  - cei/uxac066
EP  - 200
PY  - 2022///
SN  - 0009-9104
SP  - 188
TI  - Group B Streptococcus (GBS) colonisation is dynamic over time, whilst GBS capsular polysaccharides-specific antibody remains stable
T2  - Clinical and Experimental Immunology
UR  - http://dx.doi.org/10.1093/cei/uxac066
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35802786
UR  - https://academic.oup.com/cei/advance-article/doi/10.1093/cei/uxac066/6634194
UR  - http://hdl.handle.net/10044/1/98339
VL  - 209
ER  -
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