Imperial College London
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Professor Joshua B. Edel

Faculty of Natural Sciences, Department of Chemistry

Professor of Biosensing & Analytical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 0754joshua.edel Website

 
 
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Assistant

 

Mr John Murrell +44 (0)20 7594 2845

 
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Location

 

110cMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cai:2021:10.1038/s41467-021-23497-y,
author = {Cai, S and Pataillot-Meakin, T and Shibakawa, A and Ren, R and Bevan, C and Ladame, S and Ivanov, A and Edel, J},
doi = {10.1038/s41467-021-23497-y},
journal = {Nature Communications},
title = {Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum},
url = {http://dx.doi.org/10.1038/s41467-021-23497-y},
volume = {12},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures have been associated with many diseases including cancer, and miRNA profiling from liquid biopsies offers a promising strategy for cancer diagnosis, prognosis and monitoring. Here, we develop size-encoded molecular probes that can be used for simultaneous electro-optical nanopore sensing of miRNAs, allowing for ultrasensitive, sequence-specific and multiplexed detection directly in unprocessed human serum, in sample volumes as small as 0.1 μl. We show that this approach allows for femtomolar sensitivity and single-base mismatch selectivity. We demonstrate the ability to simultaneously monitor miRNAs (miR-141-3p and miR-375-3p) from prostate cancer patients with active disease and in remission. This technology can pave the way for next generation of minimally invasive diagnostic and companion diagnostic tests for cancer.
AU  - Cai,S
AU  - Pataillot-Meakin,T
AU  - Shibakawa,A
AU  - Ren,R
AU  - Bevan,C
AU  - Ladame,S
AU  - Ivanov,A
AU  - Edel,J
DO  - 10.1038/s41467-021-23497-y
PY  - 2021///
SN  - 2041-1723
TI  - Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-021-23497-y
UR  - http://hdl.handle.net/10044/1/89711
VL  - 12
ER  -
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