Imperial College London

Dr Joshua Wong

Faculty of MedicineDepartment of Infectious Disease

Clinical Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5300joshua.wong

 
 
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Location

 

210Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mullineaux-Sanders:2017:10.1016/j.celrep.2017.11.086,
author = {Mullineaux-Sanders, C and Colins, JW and Ruano-Gallego, D and Levy, M and Pevsner-Fischer, M and Glegola-Madejska, IT and Sagfors, AM and Wong, JLC and Elinav, E and Crepin, VF and Frankel, GM},
doi = {10.1016/j.celrep.2017.11.086},
journal = {Cell Reports},
pages = {3381--3389},
title = {Citrobacter rodentium relies on commensals for colonization of the colonic mucosa},
url = {http://dx.doi.org/10.1016/j.celrep.2017.11.086},
volume = {21},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - We investigated the role of commensals at the peak of infection with the colonic mouse pathogen Citrobacter rodentium. Bioluminescent and kanamycin (Kan)-resistant C. rodentium persisted avirulently in the cecal lumen of mice continuously treated with Kan. A single Kan treatment was sufficient to displace C. rodentium from the colonic mucosa, a phenomenon not observed following treatment with vancomycin (Van) or metronidazole (Met). Kan, Van, and Met induce distinct dysbiosis, suggesting C. rodentium relies on specific commensals for colonic colonization. Expression of the master virulence regulator ler is induced in germ-free mice, yet C. rodentium is only seen in the cecal lumen. Moreover, in conventional mice, a single Kan treatment was sufficient to displace C. rodentium constitutively expressing Ler from the colonic mucosa. These results show that expression of virulence genes is not sufficient for colonization of the colonic mucosa and that commensals are essential for a physiological infection course.
AU - Mullineaux-Sanders,C
AU - Colins,JW
AU - Ruano-Gallego,D
AU - Levy,M
AU - Pevsner-Fischer,M
AU - Glegola-Madejska,IT
AU - Sagfors,AM
AU - Wong,JLC
AU - Elinav,E
AU - Crepin,VF
AU - Frankel,GM
DO - 10.1016/j.celrep.2017.11.086
EP - 3389
PY - 2017///
SN - 2211-1247
SP - 3381
TI - Citrobacter rodentium relies on commensals for colonization of the colonic mucosa
T2 - Cell Reports
UR - http://dx.doi.org/10.1016/j.celrep.2017.11.086
UR - http://hdl.handle.net/10044/1/54614
VL - 21
ER -