Imperial College London
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DrJuliaSanchez Garrido

Faculty of Natural Sciences, Department of Life Sciences

Research Laboratory Manager
 
 
 
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Contact

 

julia.sanchez-garrido08

 
 
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Location

 

1.40Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Eleftheriadou:2017:10.1016/j.biomaterials.2017.01.023,
author = {Eleftheriadou, I and Dieringer, M and Poh, XY and Sanchez, -Garrido J and Gao, Y and Sgourou, A and Simmons, LE and Mazarakis, ND},
doi = {10.1016/j.biomaterials.2017.01.023},
journal = {Biomaterials},
pages = {1--14},
title = {Selective transduction of astrocytic and neuronal CNS subpopulations by lentiviral vectors pseudotyped with Chikungunya virus envelope},
url = {http://dx.doi.org/10.1016/j.biomaterials.2017.01.023},
volume = {123},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Lentiviral vectors are gene delivery vehicles that integrate into the host genome of dividing and non-dividing mammalian cells facilitating long-term transgene expression. Lentiviral vector versatility is greatly increased by incorporating heterologous viral envelope proteins onto the vector particles instead of the native envelope, conferring on these pseudotyped vectors a modified tropism and host range specificity. We investigated the pseudotyping efficiency of HIV-1 based lentiviral vectors with alphaviral envelope proteins from the Chikungunya Virus (CHIKV-G) and Sindbis Virus (SINV-G). Following vector production optimisation, titres for the CHIKV-G pseudotype were comparable to the VSV-G pseudotype but those for the SINV-G pseudotype were significantly lower. High titre CHIKV-G pseudotyped vector efficiently transduced various human and mouse neural cell lines and normal human astrocytes (NHA) in vitro. Although transduction was broad, tropism for NHAs was observed. In vivo stereotaxic delivery in striatum, thalamus and hippocampus respectively in the adult rat brain revealed localised transduction restricted to striatal astrocytes and hippocampal dentate granule neurons. Transduction of different subtypes of granule neurons from precursor to post-mitotic stages of differentiation was evident in the sub-granular zone and dentate granule cell layer. No significant inflammatory response was observed, but comparable to that of VSV-G pseudotyped lentiviral vectors. Robust long-term expression followed for three months post-transduction along with absence of neuroinflammation, coupled to the selective and unique neuron/glial tropism indicates that these vectors could be useful for modelling and gene therapy studies in the CNS.
AU  - Eleftheriadou,I
AU  - Dieringer,M
AU  - Poh,XY
AU  - Sanchez,-Garrido J
AU  - Gao,Y
AU  - Sgourou,A
AU  - Simmons,LE
AU  - Mazarakis,ND
DO  - 10.1016/j.biomaterials.2017.01.023
EP  - 14
PY  - 2017///
SN  - 1878-5905
SP  - 1
TI  - Selective transduction of astrocytic and neuronal CNS subpopulations by lentiviral vectors pseudotyped with Chikungunya virus envelope
T2  - Biomaterials
UR  - http://dx.doi.org/10.1016/j.biomaterials.2017.01.023
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000395597800001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/46244
VL  - 123
ER  -
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