Imperial College London
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ProfessorJulianGriffin

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3220julian.griffin

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hinz:2019:10.1021/acs.analchem.8b04615,
author = {Hinz, C and Liggi, S and Mocciaro, G and Jung, S and Induruwa, I and Pereira, M and Bryant, CE and Meckelmann, SW and O'Donnell, VB and Farndale, RW and Fjeldsted, J and Griffin, JL},
doi = {10.1021/acs.analchem.8b04615},
journal = {Analytical Chemistry},
pages = {8025--8035},
title = {A comprehensive UHPLC ion mobility quadrupole time-of-flight method for profiling and quantification of eicosanoids, other oxylipins, and fatty acids},
url = {http://dx.doi.org/10.1021/acs.analchem.8b04615},
volume = {91},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Analysis of oxylipins by liquid chromatography mass spectrometry (LC/MS) is challenging because of the small mass range occupied by this diverse lipid class, the presence of numerous structural isomers, and their low abundance in biological samples. Although highly sensitive LC/MS/MS methods are commonly used, further separation is achievable by using drift tube ion mobility coupled with high-resolution mass spectrometry (DTIM-MS). Herein, we present a combined analytical and computational method for the identification of oxylipins and fatty acids. We use a reversed-phase LC/DTIM-MS workflow able to profile and quantify (based on chromatographic peak area) the oxylipin and fatty acid content of biological samples while simultaneously acquiring full scan and product ion spectra. The information regarding accurate mass, collision-cross-section values in nitrogen (DTCCSN2), and retention times of the species found are compared to an internal library of lipid standards as well as the LIPID MAPS Structure Database by using specifically developed processing tools. Features detected within the DTCCSN2 and m/z ranges of the analyzed standards are flagged as oxylipin-like species, which can be further characterized using drift-time alignment of product and precursor ions distinctive of DTIM-MS. This not only helps identification by reducing the number of annotations from LIPID MAPS but also guides discovery studies of potentially novel species. Testing the methodology on Salmonella enterica serovar Typhimurium-infected murine bone-marrow-derived macrophages and thrombin activated human platelets yields results in agreement with literature. This workflow has also annotated features as potentially novel oxylipins, confirming its ability in providing further insights into lipid analysis of biological samples.
AU  - Hinz,C
AU  - Liggi,S
AU  - Mocciaro,G
AU  - Jung,S
AU  - Induruwa,I
AU  - Pereira,M
AU  - Bryant,CE
AU  - Meckelmann,SW
AU  - O'Donnell,VB
AU  - Farndale,RW
AU  - Fjeldsted,J
AU  - Griffin,JL
DO  - 10.1021/acs.analchem.8b04615
EP  - 8035
PY  - 2019///
SN  - 0003-2700
SP  - 8025
TI  - A comprehensive UHPLC ion mobility quadrupole time-of-flight method for profiling and quantification of eicosanoids, other oxylipins, and fatty acids
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.8b04615
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000474477900010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/75273
VL  - 91
ER  -
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