Imperial College London
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ProfessorJulianGriffin

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3220julian.griffin

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2020:10.1021/acs.jproteome.9b00894,
author = {Liu, K-D and Acharjee, A and Hinz, C and Liggi, S and Murgia, A and Denes, J and Gulston, MK and Wang, X and Chu, Y and West, JA and Glen, RC and Roberts, LD and Murray, AJ and Griffin, JL},
doi = {10.1021/acs.jproteome.9b00894},
journal = {Journal of Proteome Research},
pages = {3919--3935},
title = {Consequences of lipid remodeling of adipocyte membranes being functionally distinct from lipid storage in obesity.},
url = {http://dx.doi.org/10.1021/acs.jproteome.9b00894},
volume = {19},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Obesity is a complex disorder where the genome interacts with diet and environmental factors to ultimately influence body mass, composition, and shape. Numerous studies have investigated how bulk lipid metabolism of adipose tissue changes with obesity and, in particular, how the composition of triglycerides (TGs) changes with increased adipocyte expansion. However, reflecting the analytical challenge posed by examining non-TG lipids in extracts dominated by TGs, the glycerophospholipid composition of cell membranes has been seldom investigated. Phospholipids (PLs) contribute to a variety of cellular processes including maintaining organelle functionality, providing an optimized environment for membrane-associated proteins, and acting as pools for metabolites (e.g. choline for one-carbon metabolism and for methylation of DNA). We have conducted a comprehensive lipidomic study of white adipose tissue in mice which become obese either through genetic modification (ob/ob), diet (high fat diet), or a combination of the two, using both solid phase extraction and ion mobility to increase coverage of the lipidome. Composition changes in seven classes of lipids (free fatty acids, diglycerides, TGs, phosphatidylcholines, lyso-phosphatidylcholines, phosphatidylethanolamines, and phosphatidylserines) correlated with perturbations in one-carbon metabolism and transcriptional changes in adipose tissue. We demonstrate that changes in TGs that dominate the overall lipid composition of white adipose tissue are distinct from diet-induced alterations of PLs, the predominant components of the cell membranes. PLs correlate better with transcriptional and one-carbon metabolism changes within the cell, suggesting that the compositional changes that occur in cell membranes during adipocyte expansion have far-reaching functional consequences. Data are available at MetaboLights under the submission number: MTBLS1775.
AU  - Liu,K-D
AU  - Acharjee,A
AU  - Hinz,C
AU  - Liggi,S
AU  - Murgia,A
AU  - Denes,J
AU  - Gulston,MK
AU  - Wang,X
AU  - Chu,Y
AU  - West,JA
AU  - Glen,RC
AU  - Roberts,LD
AU  - Murray,AJ
AU  - Griffin,JL
DO  - 10.1021/acs.jproteome.9b00894
EP  - 3935
PY  - 2020///
SN  - 1535-3893
SP  - 3919
TI  - Consequences of lipid remodeling of adipocyte membranes being functionally distinct from lipid storage in obesity.
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/acs.jproteome.9b00894
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32646215
UR  - https://pubs.acs.org/doi/10.1021/acs.jproteome.9b00894
UR  - http://hdl.handle.net/10044/1/82487
VL  - 19
ER  -
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