Imperial College London
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ProfessorJulianGriffin

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

+44 (0)20 7594 3220julian.griffin

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tronci:2021:10.3390/antiox10050809,
author = {Tronci, L and Serreli, G and Piras, C and Frau, DV and Dettori, T and Deiana, M and Murgia, F and Santoru, ML and Spada, M and Leoni, VP and Griffin, JL and Vanni, R and Atzori, L and Caria, P},
doi = {10.3390/antiox10050809},
journal = {Antioxidants},
title = {Vitamin c cytotoxicity and its effects in redox homeostasis and energetic metabolism in papillary thyroid carcinoma cell lines},
url = {http://dx.doi.org/10.3390/antiox10050809},
volume = {10},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - High-dose of vitamin C (L-ascorbic acid, ascorbate) exhibits anti-tumoral effects, primarily mediated by pro-oxidant mechanisms. This cytotoxic effect is thought to affect the reciprocal crosstalk between redox balance and cell metabolism in different cancer types. Vitamin C also inhibits the growth of papillary thyroid carcinoma (PTC) cells, although the metabolic and redox effects remain to be fully understood. To shed light on these aspects, PTC-derived cell lines harboring the most common genetic alterations characterizing this tumor were used. Cell viability, apoptosis, and the metabolome were explored by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT), flow cytometry, and UHPLC/MS. Changes were observed in redox homeostasis, with increased reactive oxygen species (ROS) level and perturbation in antioxidants and electron carriers, leading to cell death by both apoptosis and necrosis. The oxidative stress contributed to the metabolic alterations in both glycolysis and TCA cycle. Our results confirm the pro-oxidant effect of vitamin C as relevant in triggering the cytotoxicity in PTC cells and suggest that inhibition of glycolysis and alteration of TCA cycle via NAD+ depletion can play an important role in this mechanism of PTC cancer cell death.
AU  - Tronci,L
AU  - Serreli,G
AU  - Piras,C
AU  - Frau,DV
AU  - Dettori,T
AU  - Deiana,M
AU  - Murgia,F
AU  - Santoru,ML
AU  - Spada,M
AU  - Leoni,VP
AU  - Griffin,JL
AU  - Vanni,R
AU  - Atzori,L
AU  - Caria,P
DO  - 10.3390/antiox10050809
PY  - 2021///
TI  - Vitamin c cytotoxicity and its effects in redox homeostasis and energetic metabolism in papillary thyroid carcinoma cell lines
T2  - Antioxidants
UR  - http://dx.doi.org/10.3390/antiox10050809
VL  - 10
ER  -
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