Imperial College London
Portrait Picture

Dr Joseph van Batenburg-Sherwood

Faculty of Engineering, Department of Bioengineering

Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7244jvbsherwood Website

 
 
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Location

 

Uren 416ASir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cassidy:2021:10.1016/j.omtm.2020.10.022,
author = {Cassidy, PS and Kelly, RA and Reina-Torres, E and Sherwood, JM and Humphries, MM and Kiang, A-S and Farrar, GJ and OBrien, C and Campbell, M and Stamer, WD and Overby, DR and Humphries, P and OCallaghan, J},
doi = {10.1016/j.omtm.2020.10.022},
journal = {Molecular Therapy - Methods & Clinical Development},
pages = {86--94},
title = {siRNA targeting Schlemm’s canal endothelial tight junctions enhances outflow facility and reduces IOP in a steroid-induced OHT rodent model},
url = {http://dx.doi.org/10.1016/j.omtm.2020.10.022},
volume = {20},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Systemic or localized application of glucocorticoids (GCs) can lead to iatrogenic ocular hypertension, which is a leading cause of secondary open-angle glaucoma and visual impairment. Previous work has shown that dexamethasone increases zonula occludens-1 (ZO-1) protein expression in trabecular meshwork (TM) cells, and that an antisense oligonucleotide inhibitor of ZO-1 can abolish the dexamethasone-induced increase in trans-endothelial flow resistance in cultured Schlemm’s canal (SC) endothelial and TM cells. We have previously shown that intracameral inoculation of small interfering RNA (siRNA) targeting SC endothelial cell tight junction components, ZO-1 and tricellulin, increases aqueous humor outflow facility ex vivo in normotensive mice by reversibly opening SC endothelial paracellular pores. In this study, we show that targeted siRNA downregulation of these SC endothelial tight junctions reduces intraocular pressure (IOP) in vivo, with a concomitant increase in conventional outflow facility in a well-characterized chronic steroid-induced mouse model of ocular hypertension, thus representing a potential focused clinical application for this therapy in a sight-threatening scenario.
AU  - Cassidy,PS
AU  - Kelly,RA
AU  - Reina-Torres,E
AU  - Sherwood,JM
AU  - Humphries,MM
AU  - Kiang,A-S
AU  - Farrar,GJ
AU  - OBrien,C
AU  - Campbell,M
AU  - Stamer,WD
AU  - Overby,DR
AU  - Humphries,P
AU  - OCallaghan,J
DO  - 10.1016/j.omtm.2020.10.022
EP  - 94
PY  - 2021///
SN  - 2329-0501
SP  - 86
TI  - siRNA targeting Schlemm’s canal endothelial tight junctions enhances outflow facility and reduces IOP in a steroid-induced OHT rodent model
T2  - Molecular Therapy - Methods & Clinical Development
UR  - http://dx.doi.org/10.1016/j.omtm.2020.10.022
UR  - https://www.sciencedirect.com/science/article/pii/S2329050120302266?via%3Dihub
UR  - http://hdl.handle.net/10044/1/85222
VL  - 20
ER  -
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