Imperial College London
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Dr Joseph van Batenburg-Sherwood

Faculty of Engineering, Department of Bioengineering

Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7244jvbsherwood Website

 
 
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Location

 

Uren 416ASir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhu:2021:10.1016/j.isci.2021.102042,
author = {Zhu, W and Hou, F and Fang, J and Fard, MRB and Liu, Y and Ren, S and Wu, S and Qi, Y and Sui, S and Read, AT and Sherwood, JM and Zou, W and Yu, H and Zhang, J and Overby, DR and Wang, N and Ethier, CR and Wang, K},
doi = {10.1016/j.isci.2021.102042},
journal = {iScience},
pages = {1--32},
title = {The role of Piezo1 in conventional aqueous humor outflow dynamics},
url = {http://dx.doi.org/10.1016/j.isci.2021.102042},
volume = {24},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Controlling intraocular pressure (IOP) remains the mainstay of glaucoma therapy. The trabecular meshwork (TM), the key tissue responsible for aqueous humor (AH) outflow and IOP maintenance, is very sensitive to mechanical forces. However, it is not understood whether Piezo channels, very sensitive mechanosensors, functionally influence AH outflow. Here, we characterize the role of Piezo1 in conventional AH outflow. Immunostaining and western blot analysis showed that Piezo1 is widely expressed by TM. Patch-clamp recordings in TM cells confirmed the activation of Piezo1-derived mechanosensitive currents. Importantly, the antagonist GsMTx4 for mechanosensitive channels significantly decreased steady-state facility, yet activation of Piezo1 by the specific agonist Yoda1 did not lead to a facility change. Furthermore, GsMTx4, but not Yoda1, caused a significant increase in ocular compliance, a measure of the eye's transient response to IOP perturbation. Our findings demonstrate a potential role for Piezo1 in conventional outflow, likely under pathological and rapid transient conditions.
AU  - Zhu,W
AU  - Hou,F
AU  - Fang,J
AU  - Fard,MRB
AU  - Liu,Y
AU  - Ren,S
AU  - Wu,S
AU  - Qi,Y
AU  - Sui,S
AU  - Read,AT
AU  - Sherwood,JM
AU  - Zou,W
AU  - Yu,H
AU  - Zhang,J
AU  - Overby,DR
AU  - Wang,N
AU  - Ethier,CR
AU  - Wang,K
DO  - 10.1016/j.isci.2021.102042
EP  - 32
PY  - 2021///
SN  - 2589-0042
SP  - 1
TI  - The role of Piezo1 in conventional aqueous humor outflow dynamics
T2  - iScience
UR  - http://dx.doi.org/10.1016/j.isci.2021.102042
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000621266700017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.sciencedirect.com/science/article/pii/S2589004221000109?via%3Dihub
UR  - http://hdl.handle.net/10044/1/101357
VL  - 24
ER  -
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