Imperial College London
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Dr Kieran Bates

Faculty of Medicine, School of Public Health

Visiting Researcher
 
 
 
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Location

 

VC9Variety Club WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dillon:2016:10.1111/1751-7915.12464,
author = {Dillon, MJ and Bowkett, AE and Bungard, MJ and Beckman, KM and O'Brien, MF and Bates, K and Fisher, MC and Stevens, JR and Thornton, CR},
doi = {10.1111/1751-7915.12464},
journal = {Microbial Biotechnology},
pages = {381--394},
title = {Tracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans using a highly specific monoclonal antibody and lateral- flow technology},
url = {http://dx.doi.org/10.1111/1751-7915.12464},
volume = {10},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a lethal epizootic disease of amphibians. Rapid identification of the pathogen and biosecurity is essential to prevent its spread, but current laboratory-based tests are time-consuming and require specialist equipment. Here, we describe the generation of an IgM monoclonal antibody (mAb), 5C4, specific to Bd as well as the related salamander and newt pathogen Batrachochytrium salamandrivorans (Bsal). The mAb, which binds to a glycoprotein antigen present on the surface of zoospores, sporangia and zoosporangia, was used to develop a lateral-flow assay (LFA) for rapid (15 min) detection of the pathogens. The LFA detects known lineages of Bd and also Bsal, as well as the closely related fungus Homolaphlyctis polyrhiza, but does not detect a wide range of related and unrelated fungi and oomycetes likely to be present in amphibian habitats. When combined with a simple swabbing procedure, the LFA was 100% accurate in detecting the water-soluble 5C4 antigen present in skin, foot and pelvic samples from frogs, newts and salamanders naturally infected with Bd or Bsal. Our results demonstrate the potential of the portable LFA as a rapid qualitative assay for tracking these amphibian pathogens and as an adjunct test to nucleic acid-based detection methods.
AU  - Dillon,MJ
AU  - Bowkett,AE
AU  - Bungard,MJ
AU  - Beckman,KM
AU  - O'Brien,MF
AU  - Bates,K
AU  - Fisher,MC
AU  - Stevens,JR
AU  - Thornton,CR
DO  - 10.1111/1751-7915.12464
EP  - 394
PY  - 2016///
SN  - 1751-7915
SP  - 381
TI  - Tracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans using a highly specific monoclonal antibody and lateral- flow technology
T2  - Microbial Biotechnology
UR  - http://dx.doi.org/10.1111/1751-7915.12464
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000395009600016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/49210
VL  - 10
ER  -
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