Imperial College London
Alternate

Dr Kim C Jonas

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2173k.jonas

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thompson:2009:10.1677/JOE-09-0189,
author = {Thompson, IR and Chand, AN and Jonas, KC and Burrin, JM and Steinhelper, ME and Wheeler-Jones, CP and McArdle, CA and Fowkes, RC},
doi = {10.1677/JOE-09-0189},
journal = {J Endocrinol},
pages = {215--229},
title = {Molecular characterisation and functional interrogation of a local natriuretic peptide system in rodent pituitaries, alphaT3-1 and LbetaT2 gonadotroph cells.},
url = {http://dx.doi.org/10.1677/JOE-09-0189},
volume = {203},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In the pituitary, C-type natriuretic peptide (CNP) has been implicated as a gonadotroph-specific factor, yet expression of the CNP gene (Nppc) and CNP activity in gonadotrophs is poorly defined. Here, we examine the molecular expression and putative function of a local gonadotroph natriuretic peptide system. Nppc, along with all three natriuretic peptide receptors (Npr1, Npr2 and Npr3), was expressed in both alphaT3-1 and LbetaT2 cells and primary mouse pituitary tissue, yet the genes for atrial-(ANP) and B-type natriuretic peptides (Nppa and Nppb) were much less abundant. Putative processing enzymes of CNP were also expressed in alphaT3-1 cells and primary mouse pituitaries. Transcriptional analyses revealed that the proximal 50 bp of the murine Nppc promoter were sufficient for GNRH responsiveness, in an apparent protein kinase C and calcium-dependent manner. Electrophoretic mobility shift assays showed Sp1/Sp3 proteins form major complexes within this region of the Nppc promoter. CNP protein was detectable in rat anterior pituitaries, and electron microscopy detected CNP immunoreactivity in secretory granules of gonadotroph cells. Pharmacological analyses of natriuretic peptide receptor activity clearly showed ANP and CNP are potent activators of cGMP production. However, functional studies failed to reveal a role for CNP in regulating cell proliferation or LH secretion. Surprisingly, CNP potently stimulated the human glycoprotein hormone alpha-subunit promoter in LbetaT2 cells but not in alphaT3-1 cells. Collectively, these findings support a role for CNP as the major natriuretic peptide of the anterior pituitary, and for gonadotroph cells as the major source of CNP expression and site of action.
AU  - Thompson,IR
AU  - Chand,AN
AU  - Jonas,KC
AU  - Burrin,JM
AU  - Steinhelper,ME
AU  - Wheeler-Jones,CP
AU  - McArdle,CA
AU  - Fowkes,RC
DO  - 10.1677/JOE-09-0189
EP  - 229
PY  - 2009///
SP  - 215
TI  - Molecular characterisation and functional interrogation of a local natriuretic peptide system in rodent pituitaries, alphaT3-1 and LbetaT2 gonadotroph cells.
T2  - J Endocrinol
UR  - http://dx.doi.org/10.1677/JOE-09-0189
UR  - https://www.ncbi.nlm.nih.gov/pubmed/19666697
VL  - 203
ER  -
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