Imperial College London
Alternate

Dr Kim C Jonas

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Lecturer
 
 
 
//

Contact

 

+44 (0)20 7594 2173k.jonas

 
 
//

Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Thurston:2003:humrep/deg322,
author = {Thurston, LM and Norgate, DP and Jonas, KC and Gregory, L and Wood, PJ and Cooke, BA and Michael, AE},
doi = {humrep/deg322},
journal = {Hum Reprod},
pages = {1603--1612},
title = {Ovarian modulators of type 1 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF.},
url = {http://dx.doi.org/10.1093/humrep/deg322},
volume = {18},
year = {2003}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Follicular fluid (FF) contains compounds that can modulate NADP(+)-dependent oxidation of cortisol by type 1 11beta-hydroxysteroid dehydrogenase (11betaHSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11betaHSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles. METHODS: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP(+)-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer. RESULTS: Levels of the hydrophilic ovarian 11betaHSD stimuli were significantly lower (P<0.0001) and levels of the hydrophobic ovarian 11betaHSD inhibitors were significantly higher (P<0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11betaHSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9+/-0.3 versus 8.5+/-0.2, respectively; P<0.0001). CONCLUSIONS: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11betaHSD.
AU  - Thurston,LM
AU  - Norgate,DP
AU  - Jonas,KC
AU  - Gregory,L
AU  - Wood,PJ
AU  - Cooke,BA
AU  - Michael,AE
DO  - humrep/deg322
EP  - 1612
PY  - 2003///
SN  - 0268-1161
SP  - 1603
TI  - Ovarian modulators of type 1 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF.
T2  - Hum Reprod
UR  - http://dx.doi.org/10.1093/humrep/deg322
UR  - https://www.ncbi.nlm.nih.gov/pubmed/12871869
VL  - 18
ER  -
Download