Imperial College London
Alternate

Dr Kim C Jonas

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2173k.jonas

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thurston:2003:10.1677/joe.0.1780127,
author = {Thurston, LM and Chin, E and Jonas, KC and Bujalska, IJ and Stewart, PM and Abayasekara, DRE and Michael, AE},
doi = {10.1677/joe.0.1780127},
journal = {J Endocrinol},
pages = {127--135},
title = {Expression of 11beta-hydroxysteroid dehydrogenase (11betaHSD) proteins in luteinizing human granulosa-lutein cells.},
url = {http://dx.doi.org/10.1677/joe.0.1780127},
volume = {178},
year = {2003}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In a range of tIssues, cortisol is inter-converted with cortisone by 11beta-hydroxysteroid dehydrogenase (11betaHSD). To date, two isoforms of 11betaHSD have been cloned. Previous studies have shown that human granulosa cells express type 2 11betaHSD mRNA during the follicular phase of the ovarian cycle, switching to type 1 11betaHSD mRNA expression as luteinization occurs. However, it is not known whether protein expression, and 11betaHSD enzyme activities reflect this reported pattern of mRNA expression. Hence, the aims of the current study were to investigate the expression and activities of 11betaHSD proteins in luteinizing human granulosa-lutein (hGL) cells. Luteinizing hGL cells were cultured for up to 3 days with enzyme activities (11beta-dehydrogenase (11betaDH) and 11-ketosteroid reductase (11 KSR)) and protein expression (type 1 and type 2 11betaHSD) assessed on each day of culture. In Western blots, an immunopurified type 1 11betaHSD antibody recognized a band of 38 kDa in hGL cells and in human embryonic kidney (HEK) cells stably transfected with human type 1 11betaHSD. The type 2 11betaHSD antibody recognized a band of 48 kDa in HEK cells transfected with human type 2 11betaHSD cDNA but the type 2 protein was not expressed in hGL cells throughout the 3 days of culture. While the expression of type 1 11betaHSD protein increased progressively by 2.7-fold over 3 days as hGL cells luteinized, both 11betaDH and reductase activities declined (by 52.9% and 34.2%; P<0.05) over this same period. Changes in enzyme expression and activity were unaffected by the suppression of ovarian steroid synthesis.
AU  - Thurston,LM
AU  - Chin,E
AU  - Jonas,KC
AU  - Bujalska,IJ
AU  - Stewart,PM
AU  - Abayasekara,DRE
AU  - Michael,AE
DO  - 10.1677/joe.0.1780127
EP  - 135
PY  - 2003///
SN  - 0022-0795
SP  - 127
TI  - Expression of 11beta-hydroxysteroid dehydrogenase (11betaHSD) proteins in luteinizing human granulosa-lutein cells.
T2  - J Endocrinol
UR  - http://dx.doi.org/10.1677/joe.0.1780127
UR  - https://www.ncbi.nlm.nih.gov/pubmed/12844344
VL  - 178
ER  -
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