Imperial College London
Alternate

Dr Kim C Jonas

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2173k.jonas

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thurston:2002:10.1530/rep.0.1240801,
author = {Thurston, LM and Norgate, DP and Jonas, KC and Chandras, C and Kloosterboer, HJ and Cooke, BA and Michael, AE},
doi = {10.1530/rep.0.1240801},
journal = {Reproduction},
pages = {801--812},
title = {Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity in follicular fluid from gonadotrophin-stimulated assisted conception cycles.},
url = {http://dx.doi.org/10.1530/rep.0.1240801},
volume = {124},
year = {2002}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In the ovary, cortisol-cortisone interconversion is catalysed by isoforms of 11beta-hydroxysteroid dehydrogenase (11betaHSD). The objective of this study was to establish whether human follicular fluid (hFF), obtained after controlled ovarian hyperstimulation, contains paracrine modulators of 11betaHSD activity. Of 274 hFF samples tested for effects in rat kidney homogenates, 206 hFF samples significantly inhibited NADP(+)-dependent oxidation of cortisol within 1 h (by 11-67% of control 11betaHSD activity), whereas 42 hFF samples significantly stimulated 11betaHSD activity (16-210% increase relative to control). Although charcoal-stripping of hFF prevented the inhibition and potentiated the stimulation of NADP(+)-dependent cortisol oxidation in a renal homogenate, effects of individual hFF samples on NADP(+)-dependent cortisol oxidation were independent of intrafollicular progesterone concentrations. Hydrophilic fractions of hFF samples, isolated by C18 column chromatography, stimulated both the NADP(+)-dependent oxidation of cortisol (by 55+/-5%, n=98) and the NADPH-dependent reduction of cortisone (by 86+/-22%, n= 5). In contrast, the hydrophobic fractions of hFF (eluted at 65-85% methanol) inhibited both NADP(+)-dependent 11beta-dehydrogenase and NADPH-dependent 11-ketosteroid reductase activities (by 63+/-2% and 74+/-4%, respectively). None of the C18 column fractions of 50 hFF samples had any significant effect on NAD(+)-dependent 11beta-dehydrogenase activities. The hydrophobic inhibitors of NADP(H)-dependent cortisol-cortisone metabolism did not co-elute with several candidate compounds (prostaglandins E(2) and F(2alpha), cortisol, cortisone, oestradiol, testosterone, progesterone, pregnenolone or cholesterol). Hence, hFF aspirated from women undergoing controlled ovarian hyperstimulation for assisted conception contains both hydrophilic stimuli and hydrophobic inhibitors of glucocorticoid metabolism which appear to be selective for the NADP(H)-dependent, typ
AU  - Thurston,LM
AU  - Norgate,DP
AU  - Jonas,KC
AU  - Chandras,C
AU  - Kloosterboer,HJ
AU  - Cooke,BA
AU  - Michael,AE
DO  - 10.1530/rep.0.1240801
EP  - 812
PY  - 2002///
SN  - 1470-1626
SP  - 801
TI  - Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity in follicular fluid from gonadotrophin-stimulated assisted conception cycles.
T2  - Reproduction
UR  - http://dx.doi.org/10.1530/rep.0.1240801
UR  - https://www.ncbi.nlm.nih.gov/pubmed/12530918
VL  - 124
ER  -
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