Publications
48 results found
Lei K, Sooranna SR, Bennett PR, et al., 2012, Gene Networks and Cellular Functions That Are Regulated through Progesterone and Glucocorticoid Receptors in Human Myometrial Cells., REPRODUCTIVE SCIENCES, Vol: 19, Pages: 184A-185A, ISSN: 1933-7191
Lei K, Sooranna SR, Bennett PR, et al., 2012, MKP-1 and GR Mediate Progesterone-Induced COX-2 Repression in Human Myometrium., REPRODUCTIVE SCIENCES, Vol: 19, Pages: 185A-185A, ISSN: 1933-7191
Lei K, Chen L, Cryar BJ, et al., 2011, Uterine Stretch and Progesterone Action, JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, Vol: 96, Pages: E1013-E1024, ISSN: 0021-972X
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- Citations: 29
Lei K, Sooranna SR, Bennett PR, et al., 2011, Glucocorticoid Receptor Is a Key Player That Mediates Progesterone Action in Human Myometrium, REPRODUCTIVE SCIENCES, Vol: 18, Pages: 157A-158A, ISSN: 1933-7191
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- Citations: 1
Lei K, Chen L, Sooranna SR, et al., 2011, Mechanical Stretch and Progesterone Action in Human Myometrium, REPRODUCTIVE SCIENCES, Vol: 18, Pages: 297A-297A, ISSN: 1933-7191
Sooranna SR, Lei K, Bennett PR, et al., 2011, Mechanical Stretch of Cultured Human Uterine Myocytes Leads to Increased Expression of Metallothioneins and Heat Shock 70kDa Proteins, REPRODUCTIVE SCIENCES, Vol: 18, Pages: 299A-299A, ISSN: 1933-7191
Al-Sabbagh M, Fusi L, Higham J, et al., 2011, NADPH Oxidase-Derived Reactive Oxygen Species Mediate Decidualization of Human Endometrial Stromal Cells in Response to Cyclic AMP Signaling, REPRODUCTIVE SCIENCES, Vol: 18, Pages: 162A-162A, ISSN: 1933-7191
Hua R, Lei K, Herbert B, et al., 2011, Uterine Chemokine Expression in Progesterone Delayed Labour in Mice, REPRODUCTIVE SCIENCES, Vol: 18, Pages: 296A-297A, ISSN: 1933-7191
Al-Sabbagh M, Fusi L, Higham J, et al., 2011, NADPH Oxidase-Derived Reactive Oxygen Species Mediate Decidualization of Human Endometrial Stromal Cells in Response to Cyclic AMP Signaling, ENDOCRINOLOGY, Vol: 152, Pages: 730-740, ISSN: 0013-7227
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- Citations: 43
Chen L, Lei K, Sooranna SR, et al., 2010, The Effect of cAMP on PRE Promoter Activity in Presence or Absence of Progesterone, 57th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 278A-278A, ISSN: 1933-7191
Lei K, Chen L, Sooranna SR, et al., 2010, The Effect of Pro-Inflammatory Transcription Factors on Progesterone Receptor B Transactivation in Human Myometrium, 57th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 281A-282A, ISSN: 1933-7191
Lei K, Sooranna SR, Bennett PR, et al., 2010, The Effect of Cytokines and Prostaglandins on Nuclear Receptor CoRegulators in Human Myometrium, 57th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 285A-285A, ISSN: 1933-7191
Lei K, Chen L, Sooranna SR, et al., 2010, The Role of Cyclin Dependent Kinase 2 in Stretch-Induced Progesterone Receptor B Activation in Human Myometrium, 57th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 281A-281A, ISSN: 1933-7191
Zhang M, Ma J, Lei K, et al., 2010, Molecular cloning and characterization of a class II ADP ribosylation factor from the shrimp Marsupenaeus japonicus, Fish & shellfish immunology, Vol: 28, Pages: 128-133, ISSN: 1095-9947
In shrimp, small GTPases in the Ras superfamily can regulate hemolytic phagocytosis against WSSV infection. However, the ADP ribosylation factors (Arfs), also belonging to the regulatory GTP-binding proteins and playing a central role in membrane trafficking, have not been reported yet in shrimp and their relationship with WSSV infection is completely unknown to date. Here, a novel class II Arf (designated as MjArf4) was cloned and characterized from the shrimp Marsupenaeus japonicus. Like other Arfs, MjArf4 contains an N-terminal myristoylated site, a p loop, switch regions, as well as an interswitch region. In High Five cells, when MjArf4 was in its GDP-bound form, it dispersed into the whole cell, whereas in the GTP-bound form it promoted formation of a punctuate Golgi-like structure, indicating that the MjArf4 distribution was dependent on its GDP/GTP binding. After challenge with WSSV, the mRNA level of MjArf4 was up-regulated significantly as WSSV propagated. Thus, a member of the Arf family was characterized for the first time in shrimp and found to be involved in WSSV infection.
Khajani S, Sooranna SR, Lei K, et al., 2009, Regulation of Progesterone Receptor by Transcriptions Factors in Human Uterine Myocytes, 56th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 116A-117A, ISSN: 1933-7191
Chen D, He N, Lei K, et al., 2009, Genomic organization of the translationally controlled tumor protein (TCTP) gene from shrimp Marsupenaeus japonicus, Molecular biology reports, Vol: 36, Pages: 1135-1140, ISSN: 1573-4978
The shrimp translationally controlled tumor protein (TCTP) gene was previously found up-regulated in response to viral infection. In order to further understand the transcriptional regulation of TCTP gene expression under viral challenge, the 5'-flanking promoter region of the TCTP gene in shrimp Marsupenaeus japonicus (Mj-TCTP) was cloned by using genomic walking method. The sequence shows that this region contains many important potential binding sites for transcription factors, such as activator protein1 (AP1), cAMP response element binding protein (CREB), Ets like transcription factor-1 (Elk-1), suggesting its expression is highly regulated. The promoter activity of this 5'-flanking region was confirmed by fusing to the enhanced green fluorescence protein (EGFP) gene and testing in Trichoplusia ni High Five cell line. Along with the introns and exons cloned in this study, the Mj-TCTP DNA sequence was reported here as the first TCTP gene with complete genomic organization in crustaceans.
Lei K, Li F, Zhang M, et al., 2008, Difference between hemocyanin subunits from shrimp Penaeus japonicus in anti-WSSV defense, Developmental and comparative immunology, Vol: 32, Pages: 808-813, ISSN: 0145-305X
Hemocyanin, the respiratory protein of arthropods and mollusks, was found to function against bacterial and fungal invasion. In this study, we show that this protein complex could delay the infection of white spot syndrome virus (WSSV) in vivo and cloned two hemocyanin subunits genes (PjHcL and PjHcY) from the shrimp Penaeus japonicus. Further investigation of their transcriptional regulation during viral invasion showed that in contrast to subunit PjHcY, PjHcL expression could be strongly induced by WSSV infection. These findings not only reveal an important role of hemocyanin in shrimp antiviral defense but also suggest a possible discrepancy between the two subunits in shrimp innate immunity.
Luo T, Li F, Lei K, et al., 2007, Genomic organization, promoter characterization and expression profiles of an antiviral gene PmAV from the shrimp Penaeus monodon, Molecular immunology, Vol: 44, Pages: 1516-1523, ISSN: 0161-5890
Diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. PmAV is the first antiviral gene identified from the shrimp and its mRNA level is up-regulated in response to viral infection (Luo et al., 2003). The transcriptional regulation of PmAV is critical since the expression of this gene is regulated in response to viral stimuli. In this study, we show that the Penaeus monodon PmAV gene contains four exons, separated by three introns. The 5'-flanking sequence of PmAV gene consists of a typical TATA box, several potential regulatory elements, and a compound microsatellite repeat as well. Interestingly, a TATA box and several regulatory elements also exist in the first intron (intron 1). Both intron 1 and the microsatellite are involved in transcriptional regulation of the PmAV gene. PmAV promoter activity depends on the existence of intron 1 while the microsatellite in 5'-flanking sequence acts as a negative regulative element. In addition, using Real-time PCR, we found that PmAV was highly expressed in the hepatopancreas and was up-regulated from the second day post-infection with the increase of viral load. These results extend our previous findings and provide insights into the molecular regulation of PmAV gene expression, which will be helpful for shrimp viral disease control.
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