Imperial College London
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DrKevinMonahan

Faculty of Medicine, Department of Surgery & Cancer

Honorary Clinical Senior Lecturer
 
 
 
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Contact

 

k.monahan Website

 
 
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Location

 

CRUKSt Marks HospitalNorthwick Park and St Marks Site

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Summary

 

Publications

Citation

BibTex format

@article{Awasthi:2021:10.3310/eme08010,
author = {Awasthi, A and Barbour, J and Beggs, A and Bhandari, P and Blakeway, D and Brookes, M and Brown, J and Brown, M and Caldwell, G and Clokie, S and Colleypriest, B and Conlin, A and de, Silva S and de, Caestecker J and Deeks, J and Dhar, A and Dilworth, M and Fogden, E and Foley, S and Ghosh, D and Grellier, L and Hart, A and Hoque, SS and Iacucci, M and Iqbal, T and James, J and Jarvis, M and Jayaprakash, A and Keshav, S and Magill, L and Matthews, G and Mawdsley, J and McLaughlin, S and Mehta, S and Monahan, K and Morton, D and Murugesan, S and Parkes, M and Pestinger, V and Probert, C and Ramadas, A and Rettino, A and Sebastian, S and Sharma, N and Griffiths, M and Stockton, J and Subramanian, V and Suggett, N and Taniere, P and Teare, J and Verma, AM and Wallis, Y},
doi = {10.3310/eme08010},
journal = {Efficacy and Mechanism Evaluation},
pages = {1--88},
title = {Enhanced neoplasia detection in chronic ulcerative colitis: the ENDCaP-C diagnostic accuracy study},
url = {http://dx.doi.org/10.3310/eme08010},
volume = {8},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundChronic ulcerative colitis is a large bowel inflammatory condition associated with increased colorectal cancer risk over time, resulting in 1000 colectomies per year in the UK. Despite intensive colonoscopic surveillance, 50% of cases progress to invasive cancer before detection. Detecting early (precancer) molecular changes by analysing biopsies from routine colonoscopy should increase neoplasia detection.ObjectivesTo establish a deoxyribonucleic acid (DNA) marker panel associated with early neoplastic changes in ulcerative colitis patients. To develop the DNA methylation test for high-throughput analysis within the NHS. To prospectively evaluate the test within the existing colonoscopy surveillance programme.DesignModule 1 analysed 569 stored biopsies from neoplastic and non-neoplastic sites/patients using pyrosequencing for 11 genes that were previously reported to have altered promoter methylation associated with colitis-associated neoplasia. Classifiers were constructed to predict neoplasia based on gene combinations. Module 2 translated analysis to a NHS laboratory, assessing next-generation sequencing to increase speed and reduce cost. Module 3 applied the molecular classifiers within a prospective diagnostic accuracy study, in the existing ulcerative colitis surveillance programme. Comparisons were made between baseline and reference colonoscopies undertaken in a stratified patient sample 6–12 months later.SettingThirty-one UK hospitals.ParticipantsPatients with chronic ulcerative colitis, either for at least 10 years and extensive disease, or with primary sclerosing cholangitis.InterventionsAn optimised DNA methylation classifier tested on routine mucosal biopsies taken during colonoscopy.Main outcomeIdentifying ulcerative colitis patients with neoplasia.ResultsModule 1 selected five genes with specificity for neoplasia. The optimism-adjusted area under the receiver operating characteristic curve for neoplasia was 0.83 (95% confidence interv
AU  - Awasthi,A
AU  - Barbour,J
AU  - Beggs,A
AU  - Bhandari,P
AU  - Blakeway,D
AU  - Brookes,M
AU  - Brown,J
AU  - Brown,M
AU  - Caldwell,G
AU  - Clokie,S
AU  - Colleypriest,B
AU  - Conlin,A
AU  - de,Silva S
AU  - de,Caestecker J
AU  - Deeks,J
AU  - Dhar,A
AU  - Dilworth,M
AU  - Fogden,E
AU  - Foley,S
AU  - Ghosh,D
AU  - Grellier,L
AU  - Hart,A
AU  - Hoque,SS
AU  - Iacucci,M
AU  - Iqbal,T
AU  - James,J
AU  - Jarvis,M
AU  - Jayaprakash,A
AU  - Keshav,S
AU  - Magill,L
AU  - Matthews,G
AU  - Mawdsley,J
AU  - McLaughlin,S
AU  - Mehta,S
AU  - Monahan,K
AU  - Morton,D
AU  - Murugesan,S
AU  - Parkes,M
AU  - Pestinger,V
AU  - Probert,C
AU  - Ramadas,A
AU  - Rettino,A
AU  - Sebastian,S
AU  - Sharma,N
AU  - Griffiths,M
AU  - Stockton,J
AU  - Subramanian,V
AU  - Suggett,N
AU  - Taniere,P
AU  - Teare,J
AU  - Verma,AM
AU  - Wallis,Y
DO  - 10.3310/eme08010
EP  - 88
PY  - 2021///
SN  - 2050-4365
SP  - 1
TI  - Enhanced neoplasia detection in chronic ulcerative colitis: the ENDCaP-C diagnostic accuracy study
T2  - Efficacy and Mechanism Evaluation
UR  - http://dx.doi.org/10.3310/eme08010
UR  - https://www.journalslibrary.nihr.ac.uk/eme/eme08010#/abstract
UR  - http://hdl.handle.net/10044/1/86454
VL  - 8
ER  -
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