Imperial College London
Professor Kim Parker

Professor Kim Parker

Faculty of Engineering, Department of Bioengineering

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5171k.parker Website

 
 
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Location

 

4.29Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sherwood:2019:10.3389/fbioe.2019.00276,
author = {Sherwood, JM and Boazak, EM and Feola, AJ and Parker, K and Ethier, CR and Overby, DR},
doi = {10.3389/fbioe.2019.00276},
journal = {Frontiers in Bioengineering and Biotechnology},
pages = {1--15},
title = {Measurement of ocular compliance using iPerfusion},
url = {http://dx.doi.org/10.3389/fbioe.2019.00276},
volume = {7},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The pressure-volume relationship of the eye is determined by the biomechanical properties of the corneoscleral shell and is classically characterised by Friedenwald's coefficient of ocular rigidity or, alternatively, by the ocular compliance (OC), defined as dV/dP. OC is important in any situation where the volume (V) or pressure (P) of the eye is perturbed, as occurs during several physiological and pathological processes. However, accurately measuring OC is challenging, particularly in rodents. We measured OC in 24 untreated enucleated eyes from 12 C57BL/6 mice using the iPerfusion system to apply controlled pressure steps, whilst measuring the time-varying flow rate into the eye. Pressure and flow data were analysed by a “Discrete Volume” (integrating the flow trace) and “Step Response” method (fitting an analytical solution to the pressure trace). OC evaluated at 13 mmHg was similar between the two methods (Step Response, 41 [37, 46] vs. Discrete Volume, 42 [37, 48] nl/mmHg; mean [95% CI]), although the Step Response Method yielded tighter confidence bounds on individual eyes. OC was tightly correlated between contralateral eyes (R2 = 0.75, p = 0.0003). Following treatment with the cross-linking agent genipin, OC decreased by 40 [33, 47]% (p = 0.0001; N = 6, Step Response Method). Measuring OC provides a powerful tool to assess corneoscleral biomechanics in mice and other species.
AU  - Sherwood,JM
AU  - Boazak,EM
AU  - Feola,AJ
AU  - Parker,K
AU  - Ethier,CR
AU  - Overby,DR
DO  - 10.3389/fbioe.2019.00276
EP  - 15
PY  - 2019///
SN  - 2296-4185
SP  - 1
TI  - Measurement of ocular compliance using iPerfusion
T2  - Frontiers in Bioengineering and Biotechnology
UR  - http://dx.doi.org/10.3389/fbioe.2019.00276
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2019.00276/full
UR  - http://hdl.handle.net/10044/1/74559
VL  - 7
ER  -
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