Imperial College London
Alternate

Professor Karen Polizzi

Faculty of Engineering, Department of Chemical Engineering

Professor of Biotechnology
 
 
 
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Contact

 

+44 (0)20 7594 2851k.polizzi

 
 
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Location

 

411ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lai:2021:10.1021/acschembio.1c00483,
author = {Lai, H-E and Obled, AMC and Chee, SM and Morgan, RM and Lynch, R and Sharma, SV and Moore, SJ and Polizzi, KM and Goss, RJM and Freemont, PS},
doi = {10.1021/acschembio.1c00483},
journal = {ACS Chemical Biology},
pages = {2116--2123},
title = {GenoChemetic strategy for derivatization of the violacein natural product scaffold},
url = {http://dx.doi.org/10.1021/acschembio.1c00483},
volume = {11},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Natural products and their analogues are often challenging to synthesize due to their complex scaffolds and embedded functional groups. Solely relying on engineering the biosynthesis of natural products may lead to limited compound diversity. Integrating synthetic biology with synthetic chemistry allows rapid access to much more diverse portfolios of xenobiotic compounds, which may accelerate the discovery of new therapeutics. As a proof-of-concept, by supplementing an Escherichia coli strain expressing the violacein biosynthesis pathway with 5-bromo-tryptophan in vitro or tryptophan 7-halogenase RebH in vivo, six halogenated analogues of violacein or deoxyviolacein were generated, demonstrating the promiscuity of the violacein biosynthesis pathway. Furthermore, new derivatives were generated from 5-brominated violacein analogues via the Suzuki−Miyaura cross-coupling reaction directly using the crude extract without prior purification. Herein we demonstrate a flexible and rapid approach to access a diverse chemical space that can be applied to a wide range of natural product scaffolds.
AU  - Lai,H-E
AU  - Obled,AMC
AU  - Chee,SM
AU  - Morgan,RM
AU  - Lynch,R
AU  - Sharma,SV
AU  - Moore,SJ
AU  - Polizzi,KM
AU  - Goss,RJM
AU  - Freemont,PS
DO  - 10.1021/acschembio.1c00483
EP  - 2123
PY  - 2021///
SN  - 1554-8929
SP  - 2116
TI  - GenoChemetic strategy for derivatization of the violacein natural product scaffold
T2  - ACS Chemical Biology
UR  - http://dx.doi.org/10.1021/acschembio.1c00483
UR  - https://pubs.acs.org/doi/10.1021/acschembio.1c00483
UR  - http://hdl.handle.net/10044/1/91760
VL  - 11
ER  -
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