Imperial College London
Alternate

Professor Karen Polizzi

Faculty of Engineering, Department of Chemical Engineering

Professor of Biotechnology
 
 
 
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Contact

 

+44 (0)20 7594 2851k.polizzi

 
 
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Location

 

411ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lange:2021:10.1016/j.coisb.2021.100374,
author = {Lange, OJ and Polizzi, KM},
doi = {10.1016/j.coisb.2021.100374},
journal = {Current Opinion in Systems Biology},
pages = {100374--100374},
title = {Click it or stick it: covalent and non-covalent methods for protein-self assembly},
url = {http://dx.doi.org/10.1016/j.coisb.2021.100374},
volume = {28},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Protein complexes are ubiquitous in living systems and have a range of biotechnological applications. However, building protein structures from scratch can be a difficult and laborious process. Here, we review recent developments in protein self-assembly, including a range of tools for covalent and non-covalent assembly of protein structures with user-defined architectures. Key achievements in covalent protein assembly include the development of systems with fast reaction rates and nM affinities. Non-covalent assembly methods have lagged because of the complexity of natural interactions governing protein assembly; but recent developments have created modular methods that are more broadly applicable. On the horizon, we foresee an increasing role for computational protein design tools as key in cementing the role of applications, as opposed to methodology, as the main driving force of research in this field.
AU  - Lange,OJ
AU  - Polizzi,KM
DO  - 10.1016/j.coisb.2021.100374
EP  - 100374
PY  - 2021///
SN  - 2452-3100
SP  - 100374
TI  - Click it or stick it: covalent and non-covalent methods for protein-self assembly
T2  - Current Opinion in Systems Biology
UR  - http://dx.doi.org/10.1016/j.coisb.2021.100374
UR  - https://www.sciencedirect.com/science/article/pii/S2452310021000688
UR  - http://hdl.handle.net/10044/1/94942
VL  - 28
ER  -
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