Imperial College London
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DrKaiSun

Faculty of Engineering, Department of Computing

DSI Institute Manager
 
 
 
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k.sun Website

 
 
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Location

 

William Penney LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Brandsma:2018:10.1007/s11306-018-1412-2,
author = {Brandsma, J and Goss, VM and Yang, X and Bakke, PS and Caruso, M and Chanez, P and Dahlen, S-E and Fowler, SJ and Horvath, I and Krug, N and Montuschi, P and Sanak, M and Sandstrom, T and Shaw, DE and Chung, KF and Singer, F and Fleming, LJ and Sousa, AR and Pandis, I and Bansal, AT and Sterk, PJ and Djukanovic, R and Postle, AD},
doi = {10.1007/s11306-018-1412-2},
journal = {Metabolomics},
title = {Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers},
url = {http://dx.doi.org/10.1007/s11306-018-1412-2},
volume = {14},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundLung epithelial lining fluid (ELF)—sampled through sputum induction—is a medium rich in cells, proteins and lipids. However, despite its key role in maintaining lung function, homeostasis and defences, the composition and biology of ELF, especially in respect of lipids, remain incompletely understood.ObjectivesTo characterise the induced sputum lipidome of healthy adult individuals, and to examine associations between different ELF lipid phenotypes and the demographic characteristics within the study cohort.MethodsInduced sputum samples were obtained from 41 healthy non-smoking adults, and their lipid compositions analysed using a combination of untargeted shotgun and liquid chromatography mass spectrometry methods. Topological data analysis (TDA) was used to group subjects with comparable sputum lipidomes in order to identify distinct ELF phenotypes.ResultsThe induced sputum lipidome was diverse, comprising a range of different molecular classes, including at least 75 glycerophospholipids, 13 sphingolipids, 5 sterol lipids and 12 neutral glycerolipids. TDA identified two distinct phenotypes differentiated by a higher total lipid content and specific enrichments of diacyl-glycerophosphocholines, -inositols and -glycerols in one group, with enrichments of sterols, glycolipids and sphingolipids in the other. Subjects presenting the lipid-rich ELF phenotype also had significantly higher BMI, but did not differ in respect of other demographic characteristics such as age or gender.ConclusionsWe provide the first evidence that the ELF lipidome varies significantly between healthy individuals and propose that such differences are related to weight status, highlighting the potential impact of (over)nutrition on lung lipid metabolism.
AU  - Brandsma,J
AU  - Goss,VM
AU  - Yang,X
AU  - Bakke,PS
AU  - Caruso,M
AU  - Chanez,P
AU  - Dahlen,S-E
AU  - Fowler,SJ
AU  - Horvath,I
AU  - Krug,N
AU  - Montuschi,P
AU  - Sanak,M
AU  - Sandstrom,T
AU  - Shaw,DE
AU  - Chung,KF
AU  - Singer,F
AU  - Fleming,LJ
AU  - Sousa,AR
AU  - Pandis,I
AU  - Bansal,AT
AU  - Sterk,PJ
AU  - Djukanovic,R
AU  - Postle,AD
DO  - 10.1007/s11306-018-1412-2
PY  - 2018///
SN  - 1573-3882
TI  - Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers
T2  - Metabolomics
UR  - http://dx.doi.org/10.1007/s11306-018-1412-2
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000444834200006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/63349
VL  - 14
ER  -
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