Imperial College London
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Dr Kevin Woollard

Faculty of Medicine, Department of Immunology and Inflammation

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3313 2357k.woollard Website

 
 
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Location

 

9N15CCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Normahani:2022:10.1371/journal.pone.0265393,
author = {Normahani, P and Boyle, JJ and Cave, L and Brookes, P and Woollard, KJ and Jaffer, U},
doi = {10.1371/journal.pone.0265393},
journal = {PLoS One},
pages = {1--15},
title = {Peripheral blood mononuclear cell gene expression and cytokine profiling in patients with intermittent claudication who exhibit exercise induced acute renal injury.},
url = {http://dx.doi.org/10.1371/journal.pone.0265393},
volume = {17},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Intermittent claudication (IC) is a common manifestation of peripheral arterial disease. Some patients with IC experience a rise in Urinary N-acetyl-β-D-Glucosaminidase (NAG)/ Creatinine (Cr) ratio, a marker of renal injury, following exercise. In this study, we aim to investigate whether peripheral blood mononuclear cells (PBMC) from patients with IC who exhibit a rise in urinary NAG/ Cr ratio following exercise exhibit differential IL-10/ IL-12 ratio and gene expression compared to those who do not have a rise in NAG/ Cr ratio. METHODS: We conducted a single center observational cohort study of patients diagnosed with IC. Blood and urine samples were collected at rest and following a standardised treadmill exercise protocol. For comparative analysis patients were separated into those with any rise in NAG/Cr ratio (Group 1) and those with no rise in NAG/Cr ratio (Group 2) post exercise. Isolated PBMC from pre- and post-exercise blood samples were analysed using flow cytometry. PBMC were also cultured for 20 hours to perform further analysis of IL-10 and IL-12 cytokine levels. RNA-sequencing analysis was performed to identify differentially expressed genes between the groups. RESULTS: 20 patients were recruited (Group 1, n = 8; Group 2, n = 12). We observed a significantly higher IL-10/IL-12 ratio in cell supernatant from participants in Group 1, as compared to Group 2, on exercise at 20 hours incubation; 47.24 (IQR 9.70-65.83) vs 6.13 (4.88-12.24), p = 0.04. 328 genes were significantly differentially expressed between Group 1 and 2. The modulated genes had signatures encompassing hypoxia, metabolic adaptation to starvation, inflammatory activation, renal protection, and oxidative stress. DISCUSSION: Our results suggest that some patients with IC have an altered immune status making them 'vulnerable' to systemic inflammation and renal injury following exercise. We have identified a panel of genes which are differentially expressed in this group of
AU  - Normahani,P
AU  - Boyle,JJ
AU  - Cave,L
AU  - Brookes,P
AU  - Woollard,KJ
AU  - Jaffer,U
DO  - 10.1371/journal.pone.0265393
EP  - 15
PY  - 2022///
SN  - 1932-6203
SP  - 1
TI  - Peripheral blood mononuclear cell gene expression and cytokine profiling in patients with intermittent claudication who exhibit exercise induced acute renal injury.
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0265393
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35298547
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265393
UR  - http://hdl.handle.net/10044/1/95965
VL  - 17
ER  -
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