Imperial College London
Professor Kang Li

ProfessorKangLi

Faculty of Engineering, Department of Chemical Engineering

Professor in Chemical Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5676kang.li

 
 
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Location

 

419ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Allenby:2018:2018/6230214,
author = {Allenby, MC and Tahlawi, A and Morais, JCF and Li, K and Panoskaltsis, N and Mantalaris, A},
doi = {2018/6230214},
journal = {Stem Cells International},
title = {Ceramic hollow fibre constructs for continuous perfusion and cell harvest from 3D hematopoietic organoids},
url = {http://dx.doi.org/10.1155/2018/6230214},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tissue vasculature efficiently distributes nutrients, removes metabolites, and possesses selective cellular permeability for tissue growth and function. Engineered tissue models have been limited by small volumes, low cell densities, and invasive cell extraction due to ineffective nutrient diffusion and cell-biomaterial attachment. Herein, we describe the fabrication and testing of ceramic hollow fibre membranes (HFs) able to separate red blood cells (RBCs) and mononuclear cells (MNCs) and be incorporated into 3D tissue models to improve nutrient and metabolite exchange. These HFs filtered RBCs from human umbilical cord blood (CB) suspensions of 20% RBCs to produce 90% RBC filtrate suspensions. When incorporated within 5 mL of 3D collagen-coated polyurethane porous scaffold, medium-perfused HFs maintained nontoxic glucose, lactate, pH levels, and higher cell densities over 21 days of culture in comparison to nonperfused 0.125 mL scaffolds. This hollow fibre bioreactor (HFBR) required a smaller per-cell medium requirement and operated at cell densities > 10-fold higher than current 2D methods whilst allowing for continuous cell harvest through HFs. Herein, we propose HFs to improve 3D cell culture nutrient and metabolite diffusion, increase culture volume and cell density, and continuously harvest products for translational cell therapy biomanufacturing protocols.
AU  - Allenby,MC
AU  - Tahlawi,A
AU  - Morais,JCF
AU  - Li,K
AU  - Panoskaltsis,N
AU  - Mantalaris,A
DO  - 2018/6230214
PY  - 2018///
SN  - 1687-9678
TI  - Ceramic hollow fibre constructs for continuous perfusion and cell harvest from 3D hematopoietic organoids
T2  - Stem Cells International
UR  - http://dx.doi.org/10.1155/2018/6230214
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000429639200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/60078
ER  -
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