Publications
215 results found
Willison KR, Kubota H, Hynes G, 1995, The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol, European Journal of Biochemistry, Vol: 230, Pages: 3-16
Willison KR, 1995, Folding Protein Complexes, 6,255,070
Nucleic acid encoding subunits of a polypeptide folding complex containing TCp-1 is useful in expression of the subunits for assembly of the complex in vitro or in vivo. The complex promotes folding of polypeptides, such as tubulin, e.g., following recombinant expression. The sequences share a high degree of homology but the C-termini are sufficiently different to enable peptides with sequences derived therefrom to be used in obtaining antibodies specific for the various subunits, i.e., able to distinguish between them. The antibodies may be used in analysis and design of complexes able to fold different polypeptides.
HUW LY, GOLDSBOROUGH AS, WILLISON K, et al., 1995, TCTEX2 - A SPERM TAIL SURFACE PROTEIN MAPPING TO THE T-COMPLEX, DEVELOPMENTAL BIOLOGY, Vol: 170, Pages: 183-194, ISSN: 0012-1606
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- Citations: 44
KUBOTA H, HYNES G, WILLISON K, 1995, THE CHAPERONIN CONTAINING T-COMPLEX POLYPEPTIDE-1 (TCP-1) - MULTISUBUNIT MACHINERY ASSISTING IN PROTEIN-FOLDING AND ASSEMBLY IN THE EUKARYOTIC CYTOSOL, EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol: 230, Pages: 3-16, ISSN: 0014-2956
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- Citations: 252
Kubota H, Hynes G, Willison K, 1995, The eighth Cct gene, Cctq, encoding the theta subunit of the cytosolic chaperonin containing TCP-1., Gene, Vol: 154, Pages: 231-236, ISSN: 0378-1119
The chaperonin containing t-complex polypeptide 1 (TCP-1), as one of its subunits, CCT, is a cytosolic heterooligomeric molecular chaperone assisting in the folding of proteins in eukaryotic cytosol. We have isolated a Tcp-1-related 119-bp cDNA fragment from a human cDNA library by polymerase chain reaction, and cloned full-length mouse cDNAs orthologous to the human cDNA by hybridization. The nucleotide (nt) sequence of the longest mouse clone (1844 bp) shows an open reading frame (ORF) encoding a TCP-1-related polypeptide of 548 amino acids (aa) (59,562 Da). This gene is different from Tcp-1 and the six Tcp-1-related genes reported previously, Tcp-1 (Ccta), Cctb, Cctg, Cctd, Ccte, Cctz and Ccth, which encode subunits of CCT. The product of the novel gene was analysed using an antibody raised against the C terminus of the polypeptide deduced from the nt sequence. We found that this gene encodes a subunit of CCT (polypeptide S1; 62 kDa and pI 6.25 by two-dimensional gel analysis). We have named it Cctq, encoding the theta subunit of CCT (CCT theta). The aa sequence of CCT theta shows 23-29% identity to the other CCT subunits, alpha, beta, gamma, delta, epsilon, zeta and eta, and 29% identity to the archaebacterial chaperonin TF55. CCT theta also contains the motifs common to all the other subunits of CCT which are postulated to be involved in ATPase activity.
HYNES G, KUBOTA H, WILLISON KR, 1995, ANTIBODY CHARACTERIZATION OF 2 DISTINCT CONFORMATIONS OF THE CHAPERONIN-CONTAINING TCP-1 FROM MOUSE TESTIS, FEBS LETTERS, Vol: 358, Pages: 129-132, ISSN: 0014-5793
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- Citations: 54
Kubota H, Hynes G, Willison K, 1995, The chaperonin containing t-complex polypeptide 1 (TCP-1), EJB Reviews, Publisher: Springer Berlin Heidelberg, Pages: 129-142, ISBN: 9783540609841
KUBOTA H, HYNES G, WILLISON K, 1995, THE 8TH CCT GENE, CCTQ, ENCODING THE THETA-SUBUNIT OF THE CYTOSOLIC CHAPERONIN CONTAINING TCP-1, GENE, Vol: 154, Pages: 231-236, ISSN: 0378-1119
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- Citations: 74
CREUTZ CE, LIOU A, SNYDER SL, et al., 1994, IDENTIFICATION OF THE MAJOR CHROMAFFIN GRANULE-BINDING PROTEIN, CHROMOBINDIN-A, AS THE CYTOSOLIC CHAPERONIN CCT (CHAPERONIN CONTAINING TCP-1), JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 269, Pages: 32035-32038, ISSN: 0021-9258
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- Citations: 33
KIM S, WILLISON KR, HORWICH AL, 1994, CYSTOSOLIC CHAPERONIN SUBUNITS HAVE A CONSERVED ATPASE DOMAIN BUT DIVERGED POLYPEPTIDE-BINDING DOMAINS, TRENDS IN BIOCHEMICAL SCIENCES, Vol: 19, Pages: 543-548, ISSN: 0968-0004
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- Citations: 182
Willison KR, Kubota H, 1994, The structure, function and genetics of the chaperonin containing TCP-1 (CCT) in eukaryotic cytosol, Editors: Morimoto, Tissieres, Georgopolous, Cold Spring Harbor, New York, Publisher: Cold Spring Harbor Laboratory Press, Pages: 299-312
ASHWORTH A, MALIK AN, WALKLEY NA, et al., 1994, THE TCP-1-RELATED GENE, CCTG, MAPS TO MOUSE CHROMOSOME-3, MAMMALIAN GENOME, Vol: 5, Pages: 509-510, ISSN: 0938-8990
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- Citations: 5
MIKLOS D, CAPLAN S, MERTENS D, et al., 1994, PRIMARY STRUCTURE AND FUNCTION OF A 2ND ESSENTIAL MEMBER OF THE HETEROOLIGOMERIC TCP1 CHAPERONIN COMPLEX OF YEAST, TCP1-BETA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 91, Pages: 2743-2747, ISSN: 0027-8424
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- Citations: 58
KUBOTA H, HYNES G, CARNE A, et al., 1994, IDENTIFICATION OF 6 TCP-1-RELATED GENES ENCODING DIVERGENT SUBUNITS OF THE TCP-1-CONTAINING CHAPERONIN, CURRENT BIOLOGY, Vol: 4, Pages: 89-99, ISSN: 0960-9822
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- Citations: 276
Willison KR, Horwich AL, 1993, Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1, London, Pages: 313-325
STERNLICHT H, FARR GW, STERNLICHT ML, et al., 1993, THE T-COMPLEX POLYPEPTIDE-1 COMPLEX IS A CHAPERONIN FOR TUBULIN AND ACTIN IN-VIVO, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 90, Pages: 9422-9426, ISSN: 0027-8424
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- Citations: 293
KLASSE PJ, SCHULZ TF, WILLISON KR, 1993, HIV - CYCLOPHILINS UNFOLD THE GAG, NATURE, Vol: 365, Pages: 395-396, ISSN: 0028-0836
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- Citations: 15
Willison KR, Davies PO, 1993, Molecular mechanisms of differentiation in mammalian spermatogenesis, Pages: 179-188
HARRISONLAVOIE KJ, LEWIS VA, HYNES GM, et al., 1993, A 102 KDA SUBUNIT OF A GOLGI-ASSOCIATED PARTICLE HAS HOMOLOGY TO BETA-SUBUNITS OF TRIMERIC G-PROTEINS, EMBO JOURNAL, Vol: 12, Pages: 2847-2853, ISSN: 0261-4189
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- Citations: 81
HORWICH AL, WILLISON KR, 1993, PROTEIN FOLDING IN THE CELL - FUNCTIONS OF 2 FAMILIES OF MOLECULAR CHAPERONE, HSP 60 AND TF55-TCP1, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 339, Pages: 313-326, ISSN: 0962-8436
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- Citations: 105
GOLDSBOROUGH AS, HEALY LE, COPELAND NG, et al., 1993, CLONING, CHROMOSOMAL LOCALIZATION AND EXPRESSION PATTERN OF THE POU DOMAIN GENE OCT-11, NUCLEIC ACIDS RESEARCH, Vol: 21, Pages: 127-134, ISSN: 0305-1048
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- Citations: 44
MORITA T, MURATA K, SAKAIZUMI M, et al., 1993, MOUSE T-HAPLOTYPE-SPECIFIC DOUBLE INSERTION OF B2 REPETITIVE SEQUENCES IN THE TCP-1 INTRON-7, MAMMALIAN GENOME, Vol: 4, Pages: 58-59, ISSN: 0938-8990
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- Citations: 6
KUBOTA H, WILLISON K, ASHWORTH A, et al., 1992, STRUCTURE AND EXPRESSION OF THE GENE ENCODING MOUSE T-COMPLEX POLYPEPTIDE (TCP-1), GENE, Vol: 120, Pages: 207-215, ISSN: 0378-1119
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- Citations: 30
MORITA T, KUBOTA H, MURATA K, et al., 1992, EVOLUTION OF THE MOUSE T-HAPLOTYPE - RECENT AND WORLDWIDE INTROGRESSION TO MUS-MUSCULUS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 89, Pages: 6851-6855, ISSN: 0027-8424
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- Citations: 48
LEWIS VA, HYNES GM, DONG Z, et al., 1992, T-COMPLEX POLYPEPTIDE-1 IS A SUBUNIT OF A HETEROMERIC PARTICLE IN THE EUKARYOTIC CYTOSOL, NATURE, Vol: 358, Pages: 249-252, ISSN: 0028-0836
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- Citations: 284
WILLISON K, 1991, OPPOSITE IMPRINTING OF THE MOUSE IGF2 AND IGF2R GENES, TRENDS IN GENETICS, Vol: 7, Pages: 107-109, ISSN: 0168-9525
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- Citations: 25
PLUMB M, FULTON R, BREIMER L, et al., 1991, NUCLEAR FACTOR-I ACTIVATES THE FELINE LEUKEMIA-VIRUS LONG TERMINAL REPEAT BUT IS POSTTRANSCRIPTIONALLY DOWN-REGULATED IN LEUKEMIA-CELL LINES, JOURNAL OF VIROLOGY, Vol: 65, Pages: 1991-1999, ISSN: 0022-538X
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- Citations: 27
NADEAU JH, HERRMANN B, BUCAN M, et al., 1991, GENETIC MAPS OF MOUSE CHROMOSOME-17 INCLUDING 12 NEW ANONYMOUS DNA LOCI AND 25 ANCHOR LOCI, GENOMICS, Vol: 9, Pages: 78-89, ISSN: 0888-7543
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- Citations: 53
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