Imperial College London
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DrKeittisakSuwan

Faculty of Medicine, Department of Brain Sciences

Research Associate
 
 
 
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Contact

 

keittisak.suwan

 
 
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Location

 

E404Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chongchai:2021:10.3390/v13122343,
author = {Chongchai, A and Waramit, S and Wongwichai, T and Kampangtip, J and Phitak, T and Kongtawelert, P and Hajitou, A and Suwan, K and Pothacharoen, P},
doi = {10.3390/v13122343},
journal = {Viruses},
title = {Targeting human osteoarthritic chondrocytes with ligand directed bacteriophage-based particles},
url = {http://dx.doi.org/10.3390/v13122343},
volume = {13},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Osteoarthritis (OA) is a degenerative joint disease characterized by progressive deterioration and loss of articular cartilage. There is currently no treatment to reverse the onset of OA. Thus, we developed a targeted delivery strategy to transfer genes into primary human chondrocytes as a proof-of-concept study. We displayed a chondrocyte-affinity peptide (CAP) on the pIII minor coat protein of the M13 filamentous bacteriophage (phage)-based particle carrying a mammalian transgene cassette under cytomegalovirus CMV promoter and inverted terminal repeats (ITRs) cis elements of adeno-associated virus serotype 2 (AAV-2). Primary human articular chondrocytes (HACs) were used as an in vitro model, and the selectivity and binding properties of the CAP ligand in relation to the pathogenic conditions of HACs were characterized. We found that the CAP ligand is highly selective toward pathogenic HACs. Furthermore, the stability, cytotoxicity, and gene delivery efficacy of the CAP-displaying phage (CAP.Phage) were evaluated. We found that the phage particle is stable under a wide range of temperatures and pH values, while showing no cytotoxicity to HACs. Importantly, the CAP.Phage particle, carrying a secreted luciferase (Lucia) reporter gene, efficiently and selectively delivered transgene expression to HACs. In summary, it was found that the CAP ligand preferably binds to pathogenic chondrocytes, and the CAP.Phage particle successfully targets and delivers transgene to HACs.
AU  - Chongchai,A
AU  - Waramit,S
AU  - Wongwichai,T
AU  - Kampangtip,J
AU  - Phitak,T
AU  - Kongtawelert,P
AU  - Hajitou,A
AU  - Suwan,K
AU  - Pothacharoen,P
DO  - 10.3390/v13122343
PY  - 2021///
SN  - 1999-4915
TI  - Targeting human osteoarthritic chondrocytes with ligand directed bacteriophage-based particles
T2  - Viruses
UR  - http://dx.doi.org/10.3390/v13122343
UR  - http://hdl.handle.net/10044/1/93104
VL  - 13
ER  -
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