Imperial College London
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DrKeittisakSuwan

Faculty of Medicine, Department of Brain Sciences

Research Associate
 
 
 
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Contact

 

keittisak.suwan

 
 
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Location

 

E404Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Asavarut:2022:10.15252/emmm.202115418,
author = {Asavarut, P and Waramit, S and Suwan, K and Marais, GJK and Chongchai, A and Benjathummarak, S and Al-Bahrani, M and Vila-Gomez, P and Williams, M and Kongtawelert, P and Yata, T and Hajitou, A},
doi = {10.15252/emmm.202115418},
journal = {EMBO Molecular Medicine},
pages = {1--25},
title = {Systemically targeted cancer immunotherapy and gene delivery using transmorphic particles},
url = {http://dx.doi.org/10.15252/emmm.202115418},
volume = {14},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Immunotherapy is a powerful tool for cancer treatment, but the pleiotropic nature of cytokines and immunological agents strongly limits clinical translation and safety. To address this unmet need, we designed and characterised a systemically targeted cytokine gene delivery system through transmorphic encapsidation of human recombinant adeno-associated virus DNA using coat proteins from a tumour-targeted bacteriophage (phage). We show that Transmorphic Phage/AAV (TPA) particles provide superior delivery of transgenes over current phage-derived vectors through greater diffusion across the extracellular space and improved intracellular trafficking. We used TPA to target the delivery of cytokine-encoding transgenes for interleukin-12 (IL12), and novel isoforms of IL15 and tumour necrosis factor alpha (TNFα) for tumour immunotherapy. Our results demonstrate selective and efficient gene delivery and immunotherapy against solid tumours in vivo, without harming healthy organs. Our transmorphic particle system provides a promising modality for safe and effective gene delivery, and cancer immunotherapies through cross-species complementation of two commonly used viruses.
AU  - Asavarut,P
AU  - Waramit,S
AU  - Suwan,K
AU  - Marais,GJK
AU  - Chongchai,A
AU  - Benjathummarak,S
AU  - Al-Bahrani,M
AU  - Vila-Gomez,P
AU  - Williams,M
AU  - Kongtawelert,P
AU  - Yata,T
AU  - Hajitou,A
DO  - 10.15252/emmm.202115418
EP  - 25
PY  - 2022///
SN  - 1757-4676
SP  - 1
TI  - Systemically targeted cancer immunotherapy and gene delivery using transmorphic particles
T2  - EMBO Molecular Medicine
UR  - http://dx.doi.org/10.15252/emmm.202115418
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000816755700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.embopress.org/doi/full/10.15252/emmm.202115418
UR  - http://hdl.handle.net/10044/1/104784
VL  - 14
ER  -
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