Imperial College London
Alternate

DrKirillVeselkov

Faculty of Medicine, Department of Surgery & Cancer

Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 3899kirill.veselkov04

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tillner:2017:10.1007/s13361-017-1714-z,
author = {Tillner, J and Wu, V and Jones, EA and Pringle, SD and Karancsi, T and Dannhorn, A and Veselkov, K and McKenzie, JS and Takats, Z},
doi = {10.1007/s13361-017-1714-z},
journal = {Journal of The American Society for Mass Spectrometry},
pages = {2090--2098},
title = {Faster, more reproducible DESI-MS for biological tissue imaging},
url = {http://dx.doi.org/10.1007/s13361-017-1714-z},
volume = {28},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A new, more robust sprayer for desorption electrospray ionization (DESI) mass spectrometry imaging is presented. The main source of variability in DESI is thought to be the uncontrolled variability of various geometric parameters of the sprayer, primarily the position of the solvent capillary, or more specifically, its positioning within the gas capillary or nozzle. If the solvent capillary is off-center, the sprayer becomes asymmetrical, making the geometry difficult to control and compromising reproducibility. If the stiffness, tip quality, and positioning of the capillary are improved, sprayer reproducibility can be improved by an order of magnitude. The quality of the improved sprayer and its potential for high spatial resolution imaging are demonstrated on human colorectal tissue samples by acquisition of images at pixel sizes of 100, 50, and 20 μm, which corresponds to a lateral resolution of 40-60 μm, similar to the best values published in the literature. The high sensitivity of the sprayer also allows combination with a fast scanning quadrupole time-of-flight mass spectrometer. This provides up to 30 times faster DESI acquisition, reducing the overall acquisition time for a 10 mm × 10 mm rat brain sample to approximately 1 h. Although some spectral information is lost with increasing analysis speed, the resulting data can still be used to classify tissue types on the basis of a previously constructed model. This is particularly interesting for clinical applications, where fast, reliable diagnosis is required. Graphical Abstract .
AU  - Tillner,J
AU  - Wu,V
AU  - Jones,EA
AU  - Pringle,SD
AU  - Karancsi,T
AU  - Dannhorn,A
AU  - Veselkov,K
AU  - McKenzie,JS
AU  - Takats,Z
DO  - 10.1007/s13361-017-1714-z
EP  - 2098
PY  - 2017///
SN  - 1044-0305
SP  - 2090
TI  - Faster, more reproducible DESI-MS for biological tissue imaging
T2  - Journal of The American Society for Mass Spectrometry
UR  - http://dx.doi.org/10.1007/s13361-017-1714-z
UR  - http://hdl.handle.net/10044/1/49951
VL  - 28
ER  -
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