Imperial College London

DrKonstantinosBeis

Faculty of Natural SciencesDepartment of Life Sciences

Reader in Membrane Protein Structural Biology
 
 
 
//

Contact

 

+44 (0)1235 567 809konstantinos.beis Website

 
 
//

Location

 

1.18Diamond Light Source LtdHarwell Science and Innovation Campus

//

Summary

 

Summary

MEMBRANE PROTEIN CRYSTALLOGRAPHY

Membrane proteins represent around 30% of the proteomes of most organisms and more than 40% of drug targets and yet few structures of these molecules have been solved by x-ray crystallography. Drug resistancCrystal structure of FhuA (blue and gold) in complex with MccJ25 (pink).e of bacterial pathogens is a rising crisis. Bacterial membrane proteins are essential for resistance since they are involved in the export of the drugs from the cell. My group is interested in the structural and functional characterisation of multidrug membrane transporters by X-ray crystallography.

The lab is based at the Research Complex at Harwell, Oxfordshire (https://www.rc-harwell.ac.uk/research-groups/beis-group/).

We are also interested to exploit novel antibacterials as treatments for bacterial infections by understanding how bacteria secrete antibacterial peptides and hijack membrane transporters of other bacteria to inhibit their growth under nutrient starvation.

We are also interested to understand how ABC transporters recognise their substrates as well as the conformational changes associated with ATP and substrate binding, as well as ATP hydrolysis. We employ protein crystallography, transport assays and biophysical methods such as PELDOR/EPR and smFRET.


Publications

Journals

Wong JLC, Romano M, Kerry L, et al., OmpK36-mediated Carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo, Nature Communications, ISSN:2041-1723

Beis K, Rebuffat S, 2019, Multifaceted ABC transporters associated to microcin and bacteriocin export., Res Microbiol

Pang SL, Ho KL, Waterman J, et al., 2019, Crystal structure and epitope analysis of house dust mite allergen Der f 21, Scientific Reports, Vol:9, ISSN:2045-2322

Wang L, Bateman B, Zanetti-Domingues LC, et al., 2019, Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution, Communications Biology, Vol:2, ISSN:2399-3642

Ford RC, Beis K, Learning the ABCs one at a time: structure and mechanism of ABC transporters, Biochemical Society Transactions, ISSN:0300-5127, Pages:BST20180147-BST20180147

More Publications