Imperial College London
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DrLukeAllsopp

Faculty of Medicine, National Heart & Lung Institute

Lecturer in Bacteriology of Chronic Respiratory Infection
 
 
 
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l.allsopp

 
 
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Location

 

G45Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Easton:2011:10.3389/fmicb.2011.00120,
author = {Easton, DM and Totsika, M and Allsopp, LP and Minh-Duy, P and Idris, A and Wurpel, DJ and Sherlock, O and Zhang, B and Venturini, C and Beatson, SA and Mahony, TJ and Cobbold, RN and Schembri, MA},
doi = {10.3389/fmicb.2011.00120},
journal = {Frontiers in Microbiology},
title = {Characterization of EhaJ, a new autotransporter protein from enterohemorrhagic and enteropathogenic Escherichia coli},
url = {http://dx.doi.org/10.3389/fmicb.2011.00120},
volume = {2},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) are diarrheagenic pathotypes of E. coli that cause gastrointestinal disease with the potentialfor life-threatening sequelae. While certain EHEC and EPEC virulence mechanisms havebeen extensively studied, the factors that mediate host colonization remain to be properlydefined. Previously, we identified four genes (ehaA, ehaB, ehaC, and ehaD) from the prototypic EHEC strain EDL933 that encode for proteins that belong to the autotransporter (AT)family. Here we have examined the prevalence of these genes, as well as several otherAT-encoding genes, in a collection of EHEC and EPEC strains. We show that the complement of AT-encoding genes in EHEC and EPEC strains is variable, with some AT-encodinggenes being highly prevalent. One previously uncharacterized AT-encoding gene, which wehave termed ehaJ, was identified in 12/44 (27%) of EHEC and 2/20 (10%) of EPEC strains.The ehaJ gene lies immediately adjacent to a gene encoding a putative glycosyltransferase(referred to as egtA). Western blot analysis using an EhaJ-specific antibody indicated thatEhaJ is glycosylated by EgtA. Expression of EhaJ in a recombinant E. coli strain, revealedEhaJ is located at the cell surface and in the presence of the egtA glycosyltransferasegene mediates strong biofilm formation in microtiter plate and flow cell assays. EhaJ alsomediated adherence to a range of extracellular matrix proteins, however this occurredindependent of glycosylation. We also demonstrate that EhaJ is expressed in a wild-typeEPEC strain following in vitro growth. However, deletion of ehaJ did not significantly alterits adherence or biofilm properties. In summary, EhaJ is a new glycosylated AT proteinfrom EPEC and EHEC. Further studies are required to elucidate the function of EhaJ incolonization and virulence.
AU  - Easton,DM
AU  - Totsika,M
AU  - Allsopp,LP
AU  - Minh-Duy,P
AU  - Idris,A
AU  - Wurpel,DJ
AU  - Sherlock,O
AU  - Zhang,B
AU  - Venturini,C
AU  - Beatson,SA
AU  - Mahony,TJ
AU  - Cobbold,RN
AU  - Schembri,MA
DO  - 10.3389/fmicb.2011.00120
PY  - 2011///
SN  - 1664-302X
TI  - Characterization of EhaJ, a new autotransporter protein from enterohemorrhagic and enteropathogenic Escherichia coli
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2011.00120
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000208863500130&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/71091
VL  - 2
ER  -
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