Imperial College London
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DrLukeAllsopp

Faculty of Medicine, National Heart & Lung Institute

Lecturer in Bacteriology of Chronic Respiratory Infection
 
 
 
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l.allsopp

 
 
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Location

 

G45Emmanuel Kaye BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wurpel:2015:10.1016/j.jprot.2015.11.001,
author = {Wurpel, DJ and Totsika, M and Allsopp, LP and Webb, RI and Moriel, DG and Schembri, MA},
doi = {10.1016/j.jprot.2015.11.001},
journal = {Journal of Proteomics},
pages = {177--189},
title = {Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells},
url = {http://dx.doi.org/10.1016/j.jprot.2015.11.001},
volume = {131},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Uropathogenic Escherichia coli (UPEC) are the primary cause of urinary tract infection (UTI) in humans. For the successful colonisation of the human urinary tract, UPEC employ a diverse collection of secreted or surface-exposed virulence factors including toxins, iron acquisition systems and adhesins. In this study, a comparative proteomic approach was utilised to define the UPEC pan and core surface proteome following growth in pooled human urine. Identified proteins were investigated for subcellular origin, prevalence and homology to characterised virulence factors. Fourteen core surface proteins were identified, as well as eleven iron uptake receptor proteins and four distinct fimbrial types, including type 1, P, F1C/S and a previously uncharacterised fimbrial type, designated UCA-like (UCL) fimbriae in this study. These pathogenicity island (PAI)-associated fimbriae are related to UCA fimbriae of Proteus mirabilis, associated with UPEC and exclusively found in members of the E. coli B2 and D phylogroup. We further demonstrated that UCL fimbriae promote significant biofilm formation on abiotic surfaces and mediate specific attachment to exfoliated human uroepithelial cells. Combined, this study has defined the surface proteomic profiles and core surface proteome of UPEC during growth in human urine and identified a new type of fimbriae that may contribute to UTI.
AU  - Wurpel,DJ
AU  - Totsika,M
AU  - Allsopp,LP
AU  - Webb,RI
AU  - Moriel,DG
AU  - Schembri,MA
DO  - 10.1016/j.jprot.2015.11.001
EP  - 189
PY  - 2015///
SN  - 1874-3919
SP  - 177
TI  - Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells
T2  - Journal of Proteomics
UR  - http://dx.doi.org/10.1016/j.jprot.2015.11.001
UR  - http://hdl.handle.net/10044/1/40180
VL  - 131
ER  -
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