Imperial College London
Dr Laurence Bugeon

DrLaurenceBugeon

Faculty of Natural Sciences, Department of Life Sciences

Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5405l.bugeon

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hogset:2020:10.1038/s41467-020-19827-1,
author = {Hogset, H and Horgan, C and Bergholt, M and Armstrong, J and Torraca, V and Chen, Q and Keane, TJ and Bugeon, L and Dallman, M and Mostowy, S and Stevens, M},
doi = {10.1038/s41467-020-19827-1},
journal = {Nature Communications},
pages = {1--12},
title = {In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy},
url = {http://dx.doi.org/10.1038/s41467-020-19827-1},
volume = {11},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to  access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of   biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivotemporal monitoring of the wound response in living zebrafish embryos.Overall, this study demonstrates the application of confocal Raman spectroscopic  imaging for  the comparative  bimolecular  analysis  in  fully  intact  and  living zebrafish embryos.
AU  - Hogset,H
AU  - Horgan,C
AU  - Bergholt,M
AU  - Armstrong,J
AU  - Torraca,V
AU  - Chen,Q
AU  - Keane,TJ
AU  - Bugeon,L
AU  - Dallman,M
AU  - Mostowy,S
AU  - Stevens,M
DO  - 10.1038/s41467-020-19827-1
EP  - 12
PY  - 2020///
SN  - 2041-1723
SP  - 1
TI  - In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-020-19827-1
UR  - https://www.nature.com/articles/s41467-020-19827-1
UR  - http://hdl.handle.net/10044/1/83909
VL  - 11
ER  -
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