Publications
107 results found
Mistry S, Paule CC, Varga A, et al., 2014, Prolonged exposure to bradykinin and prostaglandin E2 increases TRPV1 mRNA but does not alter TRPV1 and TRPV1b protein expression in cultured rat primary sensory neurons, NEUROSCIENCE LETTERS, Vol: 564, Pages: 89-93, ISSN: 0304-3940
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- Citations: 11
Brooke GN, Powell SM, Lavery DN, et al., 2014, Engineered repressors are potent inhibitors of androgen receptor activity, Oncotarget, Vol: 5, Pages: 959-969, ISSN: 1949-2553
Prostate cancer growth is dependent upon the Androgen Receptor (AR) pathway, hence therapies for this disease often target this signalling axis. Such therapies are successful in the majority of patients but invariably fail after a median of 2 years and tumours progress to a castrate resistant stage (CRPC). Much evidence exists to suggest that the AR remains key to CRPC growth and hence remains a valid therapeutic target. Here we describe a novel method to inhibit AR activity, consisting of an interaction motif, that binds to the AR ligand-binding domain, fused to repression domains. These ‘engineered repressors’ are potent inhibitors of AR activity and prostate cancer cell growth and importantly inhibit the AR under circumstances in which conventional therapies would be predicted to fail, such as AR mutation and altered cofactor levels.
Ottaviani S, Brooke GN, O'Hanlon-Brown C, et al., 2013, Characterisation of the androgen regulation of glycine <i>N</i>-methyltransferase in prostate cancer cells, JOURNAL OF MOLECULAR ENDOCRINOLOGY, Vol: 51, Pages: 301-312, ISSN: 0952-5041
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- Citations: 13
Lai C-F, Flach KD, Alexi X, et al., 2013, Co-regulated gene expression by oestrogen receptor α and liver receptor homolog-1 is a feature of the oestrogen response in breast cancer cells, NUCLEIC ACIDS RESEARCH, Vol: 41, Pages: 10228-10240, ISSN: 0305-1048
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- Citations: 36
Abduljabbar RF, Rakha E, Jerjees DA, et al., 2013, Liver Receptor Homolog 1 Expression and its Correlation to the Breast Biomarkers in a Large Cohort of Breast Cancer Patients, 7th Joint Meeting of the British-Division of the International-Academy-of-Pathology and the Pathological-Society-of-Great-Britain-and-Ireland, Publisher: WILEY-BLACKWELL, Pages: 20-20, ISSN: 0022-3417
Abduljabbar R, Rakha E, Jerjees D, et al., 2013, Retinoic Acid Receptor-alpha (RAR-α) is an independent prognostic marker in breast cancer and it plays different roles in ER-positive and HER2 tumours, Publisher: SPRINGER, Pages: 260-260, ISSN: 0945-6317
Pellegrino L, Stebbing J, Braga VM, et al., 2013, miR-23b regulates cytoskeletal remodeling, motility and metastasis by directly targeting multiple transcripts, Nucleic Acids Research, Vol: 41, Pages: 5400-5412, ISSN: 1362-4962
Uncontrolled cell proliferation and cytoskeletal remodeling are responsible for tumor development and ultimately metastasis. A number of studies have implicated microRNAs in the regulation of cancer cell invasion and migration. Here, we show that miR-23b regulates focal adhesion, cell spreading, cell-cell junctions and the formation of lamellipodia in breast cancer (BC), implicating a central role for it in cytoskeletal dynamics. Inhibition of miR-23b, using a specific sponge construct, leads to an increase of cell migration and metastatic spread in vivo, indicating it as a metastatic suppressor microRNA. Clinically, low miR-23b expression correlates with the development of metastases in BC patients. Mechanistically, miR-23b is able to directly inhibit a number of genes implicated in cytoskeletal remodeling in BC cells. Through intracellular signal transduction, growth factors activate the transcription factor AP-1, and we show that this in turn reduces miR-23b levels by direct binding to its promoter, releasing the pro-invasive genes from translational inhibition. In aggregate, miR-23b expression invokes a sophisticated interaction network that co-ordinates a wide range of cellular responses required to alter the cytoskeleton during cancer cell motility.
Rey J, Hu H, Kyle F, et al., 2012, Discovery of a New Class of Liver Receptor Homolog-1 (LRH-1) Antagonists: Virtual Screening, Synthesis and Biological Evaluation, CHEMMEDCHEM, Vol: 7, Pages: 1909-1914, ISSN: 1860-7179
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- Citations: 18
Ottaviani S, Brown CO, Waxman J, et al., 2012, Identification of glycine N-methyltransferase-regulated genes in prostate cancer cells, CANCER RESEARCH, Vol: 72, ISSN: 0008-5472
Baud MGJ, Leiser T, Haus P, et al., 2012, Defining the Mechanism of Action and Enzymatic Selectivity of Psammaplin A against Its Epigenetic Targets, JOURNAL OF MEDICINAL CHEMISTRY, Vol: 55, Pages: 1731-1750, ISSN: 0022-2623
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- Citations: 81
Tolhurst RS, Thomas RS, Kyle FJ, et al., 2011, Transient over-expression of estrogen receptor-α in breast cancer cells promotes cell survival and estrogen-independent growth, BREAST CANCER RESEARCH AND TREATMENT, Vol: 128, Pages: 357-368, ISSN: 0167-6806
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- Citations: 21
Thiruchelvam PTR, Lai C-F, Hua H, et al., 2011, The liver receptor homolog-1 regulates estrogen receptor expression in breast cancer cells, BREAST CANCER RESEARCH AND TREATMENT, Vol: 127, Pages: 385-396, ISSN: 0167-6806
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- Citations: 57
Ali S, Buluwela L, Coombes RC, 2011, Antiestrogens and Their Therapeutic Applications in Breast Cancer and Other Diseases, ANNUAL REVIEW OF MEDICINE, VOL 62, 2011, Vol: 62, Pages: 217-232, ISSN: 0066-4219
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- Citations: 69
Jenes A, Yan R, Buluwela L, et al., 2010, ANANDAMIDE SYNTHESIS IN PRIMARY SENSORY NEURONS, 7th Joint Meeting of the European Neuropeptide Club and the American-Summer-Neuropeptide Conference, Publisher: CHURCHILL LIVINGSTONE, Pages: 534-534, ISSN: 0143-4179
Buluwela L, Kamalati T, Photiou A, et al., 2010, A Simple Laboratory Practical to Illustrate RNA Mediated Gene Interference Using Drosophila Cell Culture, BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, Vol: 38, Pages: 393-399, ISSN: 1470-8175
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- Citations: 1
Thiruchelvam PTR, Hua H, Lai CF, et al., 2010, Characterization of estrogen responses in breast cancer cell lines highlights ERα as an LRH-1 regulated gene, Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472
Nagy B, Fedonidis C, Photiou A, et al., 2009, CAPSAICIN-SENSITIVE PRIMARY SENSORY NEURONS IN THE MOUSE EXPRESS <i>N</i>-Acyl PHOSPHATIDYLETHANOLAMINE PHOSPHOLIPASE D, NEUROSCIENCE, Vol: 161, Pages: 572-577, ISSN: 0306-4522
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- Citations: 12
Ngan S, Stronach EA, Photiou A, et al., 2009, Microarray coupled to quantitative RT-PCR analysis of androgen-regulated genes in human LNCaP prostate cancer cells, ONCOGENE, Vol: 28, Pages: 2051-2063, ISSN: 0950-9232
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- Citations: 54
Periyasamy M, Thomas R, Tolhurst R, et al., 2009, Investigation of the regulation of DNA methylation by the estrogen receptor, CANCER RESEARCH, Vol: 69, ISSN: 0008-5472
Thiruchelvam P, Photiou A, Fui LC, et al., 2009, Characterization of the nuclear receptor LRH-1 reveals a new form that can function in estrogen regulated breast cancer cell growth, CANCER RESEARCH, Vol: 69, ISSN: 0008-5472
Nagy I, Fedonidis C, Paule CC, et al., 2009, NAPE-PLD is involved in anandamide synthesis in capsaicin-sensitive primary sensory neurons, IUPS World Congress
Ali S, Periyasamy M, Lopez-Garcia J, et al., 2008, ZNF366 is a novel corepressor for estrogen receptor alpha that mediates its effects through interaction with CtBP, Breast Cancer Research Meeting, Publisher: BIOMED CENTRAL LTD, Pages: S7-S7, ISSN: 1465-5411
HART STEPHEN GB, ALI SIMAK GB, PUFONG BORIS TUMI GB, et al., 2007, CONTROL OF GENE EXPRESSION USING A COMPLEX OF AN OLIGONUCLEOTIDE AND A REGULATORY PEPTIDE, WO2002GB04633
Lopez-Garcia J, Periyasamy M, Thomas RS, et al., 2006, ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases, Nucleic Acids Research, Vol: 34, Pages: 6126-6136, ISSN: 1362-4962
The regulation of gene expression by estrogen receptor-α (ERα) requires the coordinated and temporal recruitment of diverse sets of transcriptional co-regulator complexes, which mediate nucleosome remodelling and histone modification. Using ERα as bait in a yeast two-hybrid screen, we have identified a novel ERα-interacting protein, ZNF366, which is a potent corepressor of ERα activity. The interaction between ZNF366 and ERα has been confirmed in vitro and in vivo, and is mediated by the zinc finger domains of the two proteins. Further, we show that ZNF366 acts as a corepressor by interacting with other known ERα corepressors, namely RIP140 and CtBP, to inhibit expression of estrogen-responsive genes in vivo. Together, our results indicate that ZNF366 may play an important role in regulating the expression of genes in response to estrogen.
Buluwela L, Pike J, Mazhar D, et al., 2005, Inhibiting estrogen responses in breast cancer cells using a fusion protein encoding estrogen receptor-α and the transcriptional repressor PLZF (vol 12, pg 452, 2005), GENE THERAPY, Vol: 12, Pages: 862-862, ISSN: 0969-7128
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- Citations: 1
Buluwela L, Pike J, Mazhar D, et al., 2005, Inhibiting estrogen responses in breast cancer cells using a fusion protein encoding estrogen receptor-α and the transcriptional repressor PLZF (vol 12, pg 452, 2005), GENE THERAPY, Vol: 12, Pages: 552-552, ISSN: 0969-7128
Buluwela L, Pike J, Mazhar D, et al., 2005, Inhibiting estrogen responses in breast cancer cells using a fusion protein encoding estrogen receptor-α and the transcriptional repressor PLZF, GENE THERAPY, Vol: 12, Pages: 452-460, ISSN: 0969-7128
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- Citations: 15
Lucey MJ, Chen DS, Lopez-Garcia J, et al., 2005, T:G mismatch-specific thymine-DNA glycosylase (TDG) as a coregulator of transcription interacts with SRC1 family members through a novel tyrosine repeat motif, NUCLEIC ACIDS RESEARCH, Vol: 33, Pages: 6393-6404, ISSN: 0305-1048
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- Citations: 36
Alao JP, Lam EWF, Ali S, et al., 2004, Histone deacetylase inhibitor trichostatin a represses estrogen receptor α-dependent transcription and promotes proteasomal degradation of cyclin D1 in human breast carcinoma cell lines, CLINICAL CANCER RESEARCH, Vol: 10, Pages: 8094-8104, ISSN: 1078-0432
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- Citations: 97
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