Publications
107 results found
Pike J, Holmes D, Kamalati T, et al., 2004, Silencing of androgen-regulated genes using a fusion of AR with the PLZF transcriptional repressor, ONCOGENE, Vol: 23, Pages: 7561-7570, ISSN: 0950-9232
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- Citations: 10
Buluwela L, Constantinidou D, Pike J, et al., 2004, Estrogen receptors and anti-estrogen therapies., Cancer Treat Res, Vol: 119, Pages: 271-292, ISSN: 0927-3042
Chen DS, Lucey MJ, Phoenix F, et al., 2003, T:G mismatch-specific thymine-DNA glycosylase potentiates transcription of estrogen-regulated genes through direct interaction with estrogen receptor α, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 278, Pages: 38586-38592, ISSN: 0021-9258
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- Citations: 99
Mazhar D, Ali S, Buluwela L, et al., 2003, Gene ice as applied to studies of hormonally-responsive cancers, British Cancer Research Meeting 2003, Publisher: NATURE PUBLISHING GROUP, Pages: S4-S4, ISSN: 0007-0920
Kothari MS, Ali S, Buluwela L, et al., 2003, Purified malignant mammary epithelial cells maintain hormone responsiveness in culture, British Journal of Cancer, Vol: 88, Pages: 1071-1076, ISSN: 1532-1827
Currently, the therapy for breast cancer is determined by immunohistochemical staining of the primary tumour for oestrogen receptor alpha (ERα). However, a proportion of ERα-positive patients fail to respond to tamoxifen and a proportion of ERα-negative patients show response. Here, we describe a novel procedure for the purification of malignant breast epithelial cells in an attempt to identify these patients at an early stage. Using this procedure, we are able to purify malignant cells to >90% purity as determined by immunohistochemical staining, cytology and fluorescent in situ hybridisation (FISH). While the malignant cells can be maintained in culture they do not proliferate in contrast to purified breast epithelial cells from reduction mammoplasties. Moreover, ERα and progesterone receptor (PR) expression is maintained in malignant cells, whereas normal epithelial cells rapidly lose ERα and PR. Functional studies were performed on the separated malignant cells in terms of their response to oestradiol and tamoxifen. Four out of the seven ERα-positive tumours showed a significant reduction in cell numbers after tamoxifen treatment compared to oestradiol, ERα negative tumours failed to show a response. We conclude that (a) it is possible to purify and maintain breast cancer cells for a sufficient period to permit functional studies and (b) ERα is retained in culture facilitating the use of these cells in studies of the mechanism of endocrine response and resistance in vitro.
Simak Ali, Laki Buluwela, David Holmes, et al., 2003, Silencing of endogenous gene expression, WO03010308
Schmid BC, Buluwela L, Liu QY, et al., 2002, Expression of <i>MUC</i>I splice variants correlates with invasive growth of breast cancer cell lines, BREAST CANCER RESEARCH AND TREATMENT, Vol: 76, Pages: 211-219, ISSN: 0167-6806
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- Citations: 11
Cameron-Wilson CL, Zhang H, Zhang F, et al., 2002, A vector with transcriptional terminators increases efficiency of cloning of an RNA virus by reverse transcription long polymerase chain reaction, JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, Vol: 4, Pages: 127-131, ISSN: 1464-1801
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- Citations: 5
Vigushin DM, Aboagye E, Buluwela L, et al., 2001, <i>In vivo</i> positron emission tomography imaging of estrogen-regulated gene expression in breast cancer., CLINICAL CANCER RESEARCH, Vol: 7, Pages: 3699S-3699S, ISSN: 1078-0432
Kothari MS, Tolhurst RS, Yahaya H, et al., 2001, Attenuated oestrogen receptor alpha expression following purification of normal mammary epithelial cells can be restored by adenovirus mediated gene transduction, BRITISH JOURNAL OF CANCER, Vol: 85, Pages: 45-45, ISSN: 0007-0920
Kothari MS, Slade M, Ali S, et al., 2001, Cultured purified normal breast epithelial cells rapidly lose oestrogen receptor alpha (ER) expression, whereas purified malignant breast cells stably maintain their ER status, BRITISH JOURNAL OF CANCER, Vol: 85, Pages: 45-45, ISSN: 0007-0920
Evron E, Umbricht CB, Korz D, et al., 2001, Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation, CANCER RESEARCH, Vol: 61, Pages: 2782-2787, ISSN: 0008-5472
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- Citations: 202
Loeb DM, Evron E, Patel CB, et al., 2001, Wilms' tumor suppressor gene (<i>WT1</i>) is expressed in primary breast tumors despite tumor-specific promoter methylation, CANCER RESEARCH, Vol: 61, Pages: 921-925, ISSN: 0008-5472
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- Citations: 192
Liu D, Buluwela L, All S, et al., 2001, Retroviral infection of the <i>FGF2</i> gene into MCF-7 cells induces branching morphogenesis, retards cell growth and suppresses tumorigenicity in nude mice, EUROPEAN JOURNAL OF CANCER, Vol: 37, Pages: 268-280, ISSN: 0959-8049
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- Citations: 11
Wynn SL, Fisher RA, Pagel C, et al., 2000, Organization and conservation of the <i>GART/SON/DONSON</i> locus in mouse and human genomes, GENOMICS, Vol: 68, Pages: 57-62, ISSN: 0888-7543
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- Citations: 27
Kamalati T, Niranjan B, Yant J, et al., 1999, HGF/SF in mammary epithelial growth and morphogenesis:: <i>In vitro</i> and <i>in vivo</i> models, JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, Vol: 4, Pages: 69-77, ISSN: 1083-3021
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- Citations: 29
Yant J, Buluwela L, Niranjan B, et al., 1998, <i>In vivo</i> effects of hepatocyte growth factor scatter factor on mouse mammary gland development, EXPERIMENTAL CELL RESEARCH, Vol: 241, Pages: 476-481, ISSN: 0014-4827
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- Citations: 39
Fife K, Bower M, Cooper RG, et al., 1998, Endothelial cell transfection with cationic liposomes and herpes simplex thymidine kinase mediated killing, Gene Therapy, Vol: 5, Pages: 614-620, ISSN: 0969-7128
Fife K, Buluwela L, Miller A, et al., 1998, Anti-angiogenic gene therapy, BRITISH JOURNAL OF CANCER, Vol: 78, Pages: 18-18, ISSN: 0007-0920
Yu RC, Kolettas E, Kamalati T, et al., 1997, Stable expression of CD1a molecule in human epithelial cell lines shows temperature-dependent expression and affects cell morphology and growth, ARCHIVES OF DERMATOLOGICAL RESEARCH, Vol: 289, Pages: 352-359, ISSN: 0340-3696
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- Citations: 1
Coombes RC, Buluwela L, Gomm JJ, 1997, The role of myoepithelial-derived growth factors in the human breast, 2nd International Meeting on Current Topics in Breast Cancer Research, Publisher: J ENDOCRINOLOGY LTD, Pages: 35-43, ISSN: 1351-0088
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- Citations: 1
Liu QY, Niranjan B, Gomes P, et al., 1996, Inhibitory effects of activin on the growth and morphogenesis of primary and transformed mammary epithelial cells, CANCER RESEARCH, Vol: 56, Pages: 1155-1163, ISSN: 0008-5472
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- Citations: 95
Luqmani YA, Bansal GS, Mortimer C, et al., 1996, Expression of FGFR2 BEK and K-SAM mRNA variants in normal and malignant human breast, EUROPEAN JOURNAL OF CANCER, Vol: 32A, Pages: 518-524, ISSN: 0959-8049
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- Citations: 17
NIRANJAN B, BULUWELA L, YANT J, et al., 1995, HGF/SF - A POTENT CYTOKINE FOR MAMMARY GROWTH, MORPHOGENESIS AND DEVELOPMENT, DEVELOPMENT, Vol: 121, Pages: 2897-2908, ISSN: 0950-1991
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- Citations: 153
SOTERIOU B, FISHER RA, KHAN IM, et al., 1995, CONSERVED GENE-SEQUENCES FOR SPECIES IDENTIFICATION - PCR ANALYSIS OF THE 3'-UTR OF THE SON GENE DISTINGUISHES HUMAN AND OTHER MAMMALIAN DNAS, FORENSIC SCIENCE INTERNATIONAL, Vol: 73, Pages: 171-181, ISSN: 0379-0738
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- Citations: 7
Kolettas E, Buluwela L, Bayliss MT, et al., 1995, Expression of cartilage-specific molecules is retained on long-term culture of human articular chondrocytes., J Cell Sci, Vol: 108 ( Pt 5), Pages: 1991-1999, ISSN: 0021-9533
Normal human adult articular chondrocytes were used to determine how the chondrocyte phenotype is modulated by culture conditions following long-term culture. We report here for the first time that human articular chondrocytes have a lifespan in the range of 34-37 population doublings. While chondrocytes cultured as monolayers displayed a fibroblastoid morphology and grew faster, those cultured as suspensions over agarose adopted a round morphology and formed clusters of cells reminiscent of chondrocyte differentiation in intact cartilage, with little or no DNA synthesis. These morphologies were independent of the age of the culture. Despite, these morphological differences, however, chondrocytes expressed markers at mRNA and protein levels characteristic of cartilage: namely, types II and IX collagens and the large aggregating proteoglycans, aggrecan, versican and link protein, but not syndecan, under both culture conditions. However, they also expressed type I collagen alpha 1(I) and alpha 2(I) chains. It has been suggested that expression of collagen alpha 1(I) by chondrocytes cultured as monolayers is a marker of the loss of the chondrocyte phenotype. However, we show here, using reverse transcriptase/polymerase chain reaction, that normal fresh intact human articular cartilage expresses collagen alpha 1(I). The data show that following long-term culture human articular chondrocytes retain their differentiated characteristics and that cell shape does not correlate with the expression of the chondrocyte phenotype. It is proposed that loss of the chondrocyte phenotype is marked by the loss of one or more cartilage-specific molecules rather than by the appearance of non-cartilage-specific molecules.
KOLETTAS E, BULUWELA L, BAYLISS MT, et al., 1995, EXPRESSION OF CARTILAGE-SPECIFIC MOLECULES IS RETAINED ON LONG-TERM CULTURE OF HUMAN ARTICULAR CHONDROCYTES, JOURNAL OF CELL SCIENCE, Vol: 108, Pages: 1991-1999, ISSN: 0021-9533
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- Citations: 111
GOMM JJ, BROWNE PJ, COOPE RC, et al., 1995, ISOLATION OF PURE POPULATIONS OF EPITHELIAL AND MYOEPITHELIAL CELLS FROM THE NORMAL HUMAN MAMMARY-GLAND USING IMMUNOMAGNETIC SEPARATION WITH DYNABEADS, ANALYTICAL BIOCHEMISTRY, Vol: 226, Pages: 91-99, ISSN: 0003-2697
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- Citations: 77
ATHERTON AJ, OHARE MJ, BULUWELA L, et al., 1994, ECTOENZYME REGULATION BY PHENOTYPICALLY DISTINCT FIBROBLAST SUBPOPULATIONS ISOLATED FROM THE HUMAN MAMMARY-GLAND, JOURNAL OF CELL SCIENCE, Vol: 107, Pages: 2931-2939, ISSN: 0021-9533
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- Citations: 40
KHAN IM, ZAMMIT PS, GREEN M, et al., 1994, A SIMPLE LIGATION STEP IMPROVES THE EFFICIENCY OF T-OVERHANG VECTORS, TRENDS IN GENETICS, Vol: 10, Pages: 225-226, ISSN: 0168-9525
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- Citations: 3
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