Publications
89 results found
Coppen SR, Fukushima S, Varela-Carver A, et al., 2006, Factors contributing to ventricular arrhythmias after skeletal myoblast transplantation, 79th Annual Scientific Session of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 80-80, ISSN: 0009-7322
Felkin LE, Farkasfalvi K, Soppa GKR, et al., 2006, The apelin-angiotensin receptor-like 1 (APJ) mRNA levels closely correlate with myocardial recovery in end-stage heart failure patients treated with left ventricular assist devices (LVADs), 28th Congress of the European-Society-of-Cardiology/World Congress of Cardiology, Publisher: OXFORD UNIV PRESS, Pages: 267-267, ISSN: 0195-668X
Cullen ME, Yuen AHY, Felkin LE, et al., 2006, Myocardial expression of the arginine: Glycine amidinotransferase gene is elevated in heart failure and normalized after recovery - Potential implications for local creatine synthesis, CIRCULATION, Vol: 114, Pages: I16-I20, ISSN: 0009-7322
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- Citations: 81
Felkin LE, Birks EJ, Khaghani A, et al., 2006, Gene expression profile and regulation of MMPS and TIMPS in the myocardium of patients with deteriorating heart failure requiring LVAD support compared to stable end-stage heart failure, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 25, Pages: S94-S95, ISSN: 1053-2498
Cullen ME, Yuen AH, Felkin LE, et al., 2006, Myocardial expression of the arginine:glycine aminotransferase (AGAT) gene is elevated in heart failure and normalised following post-LVAD recovery:: Potential implications for local creatine synthesis, Annual Autumn Meeting of the British-Society-for-Cardiovascular-Research, Publisher: B M J PUBLISHING GROUP, ISSN: 1355-6037
Brand NJ, Barton PJR, Felkin LE, et al., 2006, Igf-1 transcription in cardiac myocytes and fibroblasts: Relevance to heart failure, Annual Autumn Meeting of the British-Society-for-Cardiovascular-Research, Publisher: B M J PUBLISHING GROUP, ISSN: 1355-6037
Felkin LE, Birks EJ, Soppa GK, et al., 2006, Matrix metalloproteinase (MMP) and tissue inhibitor of MMPs (TIMP) gene expression in myocardial recovery from heart failure, Annual Autumn Meeting of the British-Society-for-Cardiovascular-Research, Publisher: B M J PUBLISHING GROUP, ISSN: 1355-6037
Felkin LE, Taegtmeyer AB, Barton PJR, 2006, Real-time quantitative polymerase chain reaction in cardiac transplant research., Methods Mol Biol, Vol: 333, Pages: 305-330, ISSN: 1064-3745
The real-time quantitative polymerase chain reaction (PCR), an increasingly popular technique for the detection of DNA, combines a high degree of accuracy with extreme sensitivity. In this chapter we describe the use of real-time quantitative PCR in transplantation research in two areas in which this method is commonly applied: the accurate quantification of mRNA in tissue samples and genotyping of DNA. These are described in the context of cardiac transplantation, but they are of equal relevance to other areas of transplant biology.
Cullen ME, Yuen AH, Felkin LE, et al., 2005, Myocardial expression of the arginine: Glycine aminotransferase gene is elevated in heart failure and normalized following Post-LVAD recovery: Potential implications for local creatine synthesis, 78th Annual Scientific Session of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: U461-U461, ISSN: 0009-7322
Birks EJ, Felkin LE, Taylor-Harris P, et al., 2005, Cytoskeletal protein 4.1 Isoforms in the myocardium of deteriorating patients requiring LVAD support and in myocardial recovery, 78th Annual Scientific Session of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: U460-U461, ISSN: 0009-7322
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- Citations: 3
Barton PJR, Felkin LE, Birks EJ, et al., 2005, Myocardial insulin-like growth factor-I gene expression during recovery from heart failure after combined left ventricular assist device and clenbuterol therapy, CIRCULATION, Vol: 112, Pages: I46-I50, ISSN: 0009-7322
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- Citations: 53
SantalucĂa T, Sánchez-Feutrie M, Felkin LE, et al., 2005, Phenylephrine requires the TATA box to activate transcription of GLUT1 in neonatal rat cardiac myocytes, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 38, Pages: 677-684, ISSN: 0022-2828
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- Citations: 1
De Souza AI, Felkin LE, McCormack AM, et al., 2005, Sequential expression of three known protective genes in cardiac biopsies after transplantation, TRANSPLANTATION, Vol: 79, Pages: 584-590, ISSN: 0041-1337
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- Citations: 13
Birks EJ, Felkin LE, Hardy J, et al., 2005, Changes in myocardial pro- and antiinflammatory cytokines during and one year after successful LVAD explantation, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 24, Pages: S101-S101, ISSN: 1053-2498
Taylor-Harris PM, Felkin LE, Birks EJ, et al., 2005, Expression of human membrane skeleton protein genes for protein 4.1 and βIIΣ2-spectrin assayed by real-time RT-PCR, CELLULAR & MOLECULAR BIOLOGY LETTERS, Vol: 10, Pages: 135-149, ISSN: 1425-8153
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- Citations: 16
Felkin LE, Birks EJ, Hall JL, et al., 2004, Altered gene expression of tissue inhibitors of metalloproteinases in clinical recovery of heart failure with combined left ventricular assist device and clenbuterol therapy, 77th Scientific Meeting of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 632-632, ISSN: 0009-7322
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- Citations: 1
Barton PJ, Felkin LE, Birks EJ, et al., 2004, Elevated myocardial expression of insulin-like growth factor I and related genes following clinical recovery in patients with combined left ventricular assist device and clenbuterol therapy, 77th Scientific Meeting of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 631-632, ISSN: 0009-7322
Birks EJ, Felkin LE, Hardy J, et al., 2004, Changes in myocardial cytokine expression during and after successful left-ventricular assist device explantation, ESC Congress 2004, Publisher: W B SAUNDERS CO LTD, Pages: 185-185, ISSN: 0195-668X
Barton PJR, Felkin LE, Koban MU, et al., 2004, The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart., Mol Cell Biochem, Vol: 263, Pages: 91-97, ISSN: 0300-8177
Cardiac muscle development is characterised by the activation of contractile protein genes and subsequent modulation of expression resulting, ultimately, in the formation of a mature four-chambered organ. Myocardial gene expression is also altered in the adult in response to pathological stimuli and this is thought to contribute to the altered contractile characteristics of the diseased heart. We have examined the expression of the slow skeletal troponin T (TnT) gene in the human heart during development and in disease using whole mount in situ hybridisation and real-time quantitative (TaqMan) polymerase chain reaction (PCR). Slow skeletal TnT mRNA shows transitory and regional expression in the early foetal heart, which occurs at different times in atria and ventricles. In ventricular myocardium, expression is seen in the outer epicardial layer at a time when the coronary circulation is being established. Expression was detected at low levels in the adult human heart and was significantly increased in end-stage heart failure. Similarly, expression was readily detectable during early rat heart development and was up-regulated in pressure overload hypertrophy in adult. Together these data show for the first time that slow skeletal TnT mRNA is readily detectable during early human heart development. They further suggest that slow skeletal TnT may be responsive to myocardial stress and that elevated levels may contribute to myocardial dysfunction in adult disease. (Mol Cell Biochem 263: 91-97, 2004).
Stoutamyer A, Barton PJ, Felkin LE, et al., 2004, Expression pattern of troponin C and troponin T isoforms in developing and pathological heart, 18th World Congress of the International-Society-for-Heart-Research, Publisher: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, Pages: 168-169, ISSN: 0022-2828
Birks EJ, Felkin LE, Banner NR, et al., 2004, Increased toll-like receptor 4 in the myocardium of patients requiring left ventricular assist devices, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 23, Pages: 228-235, ISSN: 1053-2498
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- Citations: 73
Barton PJR, Felkin LE, Koban MU, et al., 2004, The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart., Mol Cell Biochem, Vol: 263, Pages: 91-97
Chester AH, Azam R, Felkin L, et al., 2003, Functional consequences for alternative splicing of the endothelin a receptor gene, 76th Annual Scientific Session of the American-Heart-Association, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 20-20, ISSN: 0009-7322
Azam R, Felkin L, Brand NJ, et al., 2003, Functional consequences for alternative splicing of the ET<sub>A</sub> receptor gene, 14th Annual Symposium of the London-Hypertension-Society, Publisher: BLACKWELL PUBLISHING LTD, Pages: 244-244, ISSN: 0306-5251
Barton PJR, Birks EJ, Felkin LE, et al., 2003, Increased expression of extracellular matrix regulators TIMP1 and MMP1 in deteriorating heart failure, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 22, Pages: 738-744, ISSN: 1053-2498
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- Citations: 57
Felkin LE, Birks EJ, Yacoub MH, et al., 2003, Elevated myocardial TIMP1 expression correlates with interleukin-6 in patients with deteriorating heart failure, and is stimulated by interleukin-6 in vitro, Congress of the European-Society-of-Cardiology, Publisher: W B SAUNDERS CO LTD, Pages: 144-144, ISSN: 0195-668X
Bhavsar PK, Felkin LE, Barton PJR, et al., 2002, Molecular and morphological effects of clenbuterol on neonatal rat cardiac myocytes in culture, Congress of the European-Society-of-Cardiology, Publisher: W B SAUNDERS CO LTD, Pages: 369-369, ISSN: 0195-668X
Birks EJ, Latif N, Owen V, et al., 2001, Quantitative myocardial cytokine expression and activation of the apoptotic pathway in patients who require left ventricular assist devices, CIRCULATION, Vol: 104, Pages: I233-I240, ISSN: 0009-7322
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- Citations: 60
Birks EJ, Latif N, Owen V, et al., 2001, Quantitative myocardial cytokine expression and activation of the apoptotic pathway in patients who require left ventricular assist devices, Circulation, Vol: 104, ISSN: 0009-7322
Background - Molecular mechanisms underlying the deterioration of patients undergoing LV assist device (LVAD) implantation remain poorly understood. We studied the cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-1β and IL-6 and the terminal stage of the apoptotic pathway in patients with decompensating heart failure who required LVAD support and compared them with patients with less severe heart failure undergoing elective heart transplantation. Methods and Results - Myocardial and serum samples from 23 patients undergoing LVAD implantation were compared with those from 36 patients undergoing elective heart transplantation. Myocardial TNF-α mRNA (1.71-fold; P<0.05) and protein (3.43±0.19 versus 2.95±0.10 pg/mg protein; P<0.05) were elevated in the LVAD patients. Immunocytochemistry demonstrated TNF expression in the myocytes. Serum TNF-α was also elevated (12.5±1.9 versus 4.0±0.4 pg/mL; P<0.0001) in the LVAD patients. IL-6 mRNA (2.57-fold higher; P<0.005) and protein (27.83±9.35 versus 4.26±1.24 pg/mg protein; P<0.001) were higher in the LVAD candidates, as was serum IL-6 (79.3±23.6 versus 7.1±1.6 pg/mL; P<0.0001). Interleukin-1β mRNA expression was 9.78-fold higher in the LVAD patients (P<0.001). iNOS mRNA expression was similar to that in advanced heart failure patients and was not further elevated in the LVAD patients. Levels of procaspase-9 (8.02±0.91 versus 6.16±0.43 oligodeoxynucleotide [OD] units; P<0.01), cleaved caspase-9 (10.02±1.0 versus 7.34±0.40 OD units; P<0.05), intact and spliced DFF-45 (4.58±0.75 versus 2.84±0.23 OD units; P±0.05) were raised in LVAD patients, but caspase-3 and human nuclease CPAN were not. Conclusions - Elevated TNF-α, IL-1β, and IL-6 and alterations in the apoptotic pathway were found in the myocardium and elevated TNF-α and IL-6 in serum of de
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