Imperial College London
Alternate

Dr Lata Govada

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Research Associate
 
 
 
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Contact

 

+44 (0)20 7594 3037l.govada

 
 
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Location

 

Open Plan no 12Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Govada:2022:10.1002/adfm.202202596,
author = {Govada, L and Rubio, N and Saridakis, E and Balaskandan, K and Leese, HS and Li, Y and Wang, B and Shaffer, MSP and Chayen, N},
doi = {10.1002/adfm.202202596},
journal = {Advanced Functional Materials},
title = {Graphene-based nucleants for protein crystallization},
url = {http://dx.doi.org/10.1002/adfm.202202596},
volume = {32},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Protein crystallization remains a major bottleneck for the determination of high resolution structures. Nucleants can accelerate the process but should ideally be compatible with high throughput robotic screening. Polyethylene glycol grafted (PEGylated) graphenes can be stabilized in water providing dispensable, nucleant systems. Two graphitic feedstocks are exfoliated and functionalized with PEG using a non-destructive, scalable, chemical reduction method, delivering good water dispersibility (80 and 750 µg mL−1 for large and small layers, respectively). The wide utility of these nucleants has been established across five proteins and three different screens, each of 96 conditions, demonstrating greater effectiveness of the dispersed PEGylated graphenes. Smaller numbers of larger, more crystalline flakes consistently act as better protein nucleants. The delivered nucleant concentration is optimized (0.1 mg mL−1 in the condition), and the performance benchmarked against existing state of the art, molecularly imprinted polymer nucleants. Strikingly, graphene nucleants are effective even when decreasing both the nucleant and protein concentration to unusually low concentrations. The set-up to scale-up nucleant production to liter volumes can provide sufficient material for wide implementation. Together with the optimized crystallization conditions, the results are a step forward toward practical synthesis of a readily accessible “universal” nucleant.
AU  - Govada,L
AU  - Rubio,N
AU  - Saridakis,E
AU  - Balaskandan,K
AU  - Leese,HS
AU  - Li,Y
AU  - Wang,B
AU  - Shaffer,MSP
AU  - Chayen,N
DO  - 10.1002/adfm.202202596
PY  - 2022///
SN  - 1616-301X
TI  - Graphene-based nucleants for protein crystallization
T2  - Advanced Functional Materials
UR  - http://dx.doi.org/10.1002/adfm.202202596
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000811086100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1002/adfm.202202596
UR  - http://hdl.handle.net/10044/1/97889
VL  - 32
ER  -
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